Xylose medium suitable for ustilaginoidea virens spore production and application method thereof

A rice smut fungus and an application method technology, applied in the fields of plant pathology technology and microbiology technology, can solve the problems of multiple hyphae fragments, a decrease in the number of spores, and the inability to provide spores stably, and achieve the effect of simple and convenient operation.

Pending Publication Date: 2016-07-20
GUANGXI UNIV
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are also some obvious deficiencies in the technology of preparing spores with this medium. After the sporulation peak, a large number of non-sporulation aerial hyphae grow quickly on the plate colony, resulting in a sharp de

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Xylose medium suitable for ustilaginoidea virens spore production and application method thereof
  • Xylose medium suitable for ustilaginoidea virens spore production and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0031] The preparation of xylose culture medium of the present invention needs 2 kinds of sterilizing techniques to synthesize, and basal medium (potato agar) adopts conventional high temperature and high pressure sterilization technology; And component xylose (xylose solution) can not be through high temperature treatment, can only adopt filter Sterilization; the sterilized products of these two technologies are mixed in a non-high temperature state to become an effective sporulation medium.

[0032] After mixing the two components (potato agar component, xylose solution component), the effective concentration of all components will decrease, therefore, the concentration of the two components before mixing must be higher than the component concentration of the final medium (Potato 100g, xylose 10g, agar 20g, water 1000ml). In actual design, the concentration of the two components before mixing can be calculated according to the mixing ratio of the two components.

[0033] Th...

Embodiment 1

[0038] Adopt a kind of xylose culture medium suitable for rice smut fungus sporulation of the present invention and its application method, cultivate and prepare the parenchyma conidia of rice smut fungus bacterial strain Uv-111, carry out operation according to the following steps:

[0039]1. Preparation of basal medium

[0040] The ratio is: 100g of potatoes, 20g of agar, and 900ml of water to prepare a basal medium, 90ml in each bottle, and use it for later use after conventional high-temperature sterilization.

[0041] 2. Filter Sterilization of Xylose

[0042] The proportioning ratio is: 10g of xylose, 100ml of water, the xylose is mixed with water to form a xylose solution, and the xylose solution is filtered and sterilized with a bacterial filter under aseptic conditions, and the xylose solution is ready for use.

[0043] 3. Preparation of Xylose Medium Plates

[0044] Heat and melt the basal medium prepared in step 1, equilibrate the water bath to 60°C, and at the sa...

Embodiment 2

[0052] Adopt a kind of xylose medium suitable for rice smut fungus sporulation of the present invention and its application method, cultivate and prepare the parenchyma conidia of rice smut fungus bacterial strain Uv-110, operate and implement according to the steps 1 to 6 of embodiment 1, only The strain implanted in step 4 is Uv-110. After step 6 has been implemented and operated, wash a plate of plate colony with 15ml sterile water, and the concentration of the new spore liquid obtained is 1.35×10 6 spores / ml.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a xylose medium suitable for ustilaginoidea virens spore production and an application method thereof. A potato xylose medium is prepared from xylose after filter sterilization and serves as a spore producing medium for culturing thin-wall conidia of ustilaginoidea virens. The preparation and application method of the medium comprises the following steps: 1) preparing a basic medium; 2) performing filter sterilization of xylose; 3) preparing a xylose medium plate; 4) implanting ustilaginoidea virens; 5) performing constant-temperature culture; and 6) collecting new-generation spores. The xylose medium has the characteristics and advantages that the time for obtaining a large quantity of spores is short; the continuous spore producing time is long; the operation is simple and convenient, and shaking culture equipment is not needed; and the new-generation spores are relatively pure.

Description

technical field [0001] The present invention relates to phytopathological techniques and microbiological techniques. Specifically, it relates to a xylose medium suitable for spore production of rice smut fungus and an application method thereof. Background technique [0002] In most fungal disease systems, the propagules of pathogenic bacteria not only have the function of multiplying and expanding their own population, but more importantly, they have the function of interacting with and identifying the host, and initiating the function of infecting the host. Therefore, in many important plant disease research In the process, the propagules of pathogenic bacteria need to be used as test materials. Obviously, an efficient and convenient method for culturing and preparing propagule materials will be very conducive to the smooth development of research work. Rice false smut (caused by Ustilaginoideavirens (Cooke) Takahashi infection) has become a major disease of rice producti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N3/00
CPCC12N3/00
Inventor 张君成王忠文曾东强蒲相君杨平
Owner GUANGXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap