Xylose medium suitable for ustilaginoidea virens spore production and application method thereof

Abstract

The invention discloses a xylose medium suitable for ustilaginoidea virens spore production and an application method thereof. A potato xylose medium is prepared from xylose after filter sterilization and serves as a spore producing medium for culturing thin-wall conidia of ustilaginoidea virens. The preparation and application method of the medium comprises the following steps: 1) preparing a basic medium; 2) performing filter sterilization of xylose; 3) preparing a xylose medium plate; 4) implanting ustilaginoidea virens; 5) performing constant-temperature culture; and 6) collecting new-generation spores. The xylose medium has the characteristics and advantages that the time for obtaining a large quantity of spores is short; the continuous spore producing time is long; the operation is simple and convenient, and shaking culture equipment is not needed; and the new-generation spores are relatively pure.

Description

technical field [0001] The present invention relates to phytopathological techniques and microbiological techniques. Specifically, it relates to a xylose medium suitable for spore production of rice smut fungus and an application method thereof. Background technique [0002] In most fungal disease systems, the propagules of pathogenic bacteria not only have the function of multiplying and expanding their own population, but more importantly, they have the function of interacting with and identifying the host, and initiating the function of infecting the host. Therefore, in many important plant disease research In the process, the propagules of pathogenic bacteria need to be used as test materials. Obviously, an efficient and convenient method for culturing and preparing propagule materials will be very conducive to the smooth development of research work. Rice false smut (caused by Ustilaginoideavirens (Cooke) Takahashi infection) has become a major disease of rice producti...

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Problems solved by technology

However, there are also some obvious deficiencies in the technology of preparing spores with this medium. After the sporulation peak, a large number of non-sporulation aerial hyphae grow quickly on the plate colony, resulting in a sharp decline in the number of spores, which cannot be produced in a long period of time. Stable provision of spores for use; moreover, there are still more fragments of mycelium in the new spore liquid collected for preparation of culture results

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