Method for realizing RNA (ribonucleic acid) overexpression by ovarian orthotopic injection and application of method

An in situ injection and overexpression technology, applied in DNA/RNA fragments, recombinant DNA technology, biochemical equipment and methods, etc., can solve the problem of not finding the same or similar reports, save experimental costs, and improve expression levels. High, overexpressed good effect

Inactive Publication Date: 2016-07-20
SOUTH CHINA AGRI UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] So far no report identical or similar to the method of the present invention has been found

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for realizing RNA (ribonucleic acid) overexpression by ovarian orthotopic injection and application of method
  • Method for realizing RNA (ribonucleic acid) overexpression by ovarian orthotopic injection and application of method
  • Method for realizing RNA (ribonucleic acid) overexpression by ovarian orthotopic injection and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1).PCR primer design. PCR primers were designed for the cDNA sequence of mouse ovary lncRNA-NONMMUT047274. The primer sequences are as follows:

[0042]

[0043] 2). PCR template DNA preparation. Total RNA was extracted from the ovarian tissues of C57BL / 6 mice and cDNA templates for PCR were obtained by reverse transcription. Use TaKaRa HSDNAPolymerase enzyme amplification. After the PCR reaction, take 3 μl of the amplification product and add 1 μl of 5×DNA loading buffer to electrophoresis in 1% agarose gel at 120V, observe whether there are bright bands under ultraviolet light, and prepare for the purification of the PCR product.

[0044] 3). PCR amplification product purification. Use TaKaRaMiniBESTAgaroseGelDNAExtractionKitVer.4.0 Gel Extraction Kit for purification of PCR amplification products.

[0045] 4). PCR amplification product and MOUSEZP3PROMOTER (JD#445 backbone vector) backbone vector enzyme digestion reaction. Use NEBXhoI and SmaI restriction e...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of biology and discloses a method for realizing RNA (ribonucleic acid) overexpression by ovarian orthotopic injection and application of the method.RNA overexpression in a mouse ovary is realized by means of in-vivo transfection so as to provide a technical support for further researches on functions of RNA in the ovary. The method has the advantages that the defect of lack of targeting property of caudal vein over-expressed genes is avoided; transfection reagent consumption is reduced by dozens of times as compared with that of caudal vein injection, so that experiment cost is saved; excellent overexpression effects are achieved, and RNA expression level is raised remarkably as compared with that of a blank control group. The method which is minimally invasive has no influences on survival of mice.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method and application for realizing RNA overexpression by ovarian in situ injection. Background technique [0002] No report identical or similar to the method of the present invention has been found so far. Contents of the invention [0003] In order to overcome the shortcomings and deficiencies of the prior art, the primary purpose of the present invention is to provide a method for realizing RNA overexpression by ovarian in situ injection. The invention realizes the overexpression of RNA in the mouse ovary through the method of in vivo transfection, and provides technical support for further research on the function of RNA in the ovary. [0004] Another object of the present invention is to provide the application of the method for achieving RNA overexpression by orthotopic ovarian injection. [0005] The object of the present invention is achieved through the f...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85C12N15/66C12N15/113
CPCC12N15/113C12N15/66C12N15/85C12N2310/14C12N2800/107
Inventor 杨世华李加宇吴绮琪方志浩黄群山
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap