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Quick detecting card for immunochromatography of organophosphorus and carbamate pesticide multiresidue cholinesterase

A carbamate and pesticide residue technology, applied in the field of pesticide detection, can solve problems such as instability, high cost, and difficulty in antibody preparation

Active Publication Date: 2016-07-20
广州万联生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its poor sensitivity and stability, it has gradually withdrawn from the market, and only a few developing countries such as South Korea are still using it.
Immunoassays based on antigen-antibody specific binding reactions can also be used for the detection of some pesticides, but due to the difficulty of antibody preparation, high cost, and instability, currently only one or a small class of pesticides can be detected

Method used

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  • Quick detecting card for immunochromatography of organophosphorus and carbamate pesticide multiresidue cholinesterase
  • Quick detecting card for immunochromatography of organophosphorus and carbamate pesticide multiresidue cholinesterase
  • Quick detecting card for immunochromatography of organophosphorus and carbamate pesticide multiresidue cholinesterase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1 The structure of the quick test card of the present invention

[0059] 1. The quick test card of the present invention includes a base plate and a cover plate, and the base plate includes at least two groups of buffer pads, sample pads, enzyme pads, detection membranes, and water-absorbing pads that are pasted in sequence; one side of the buffer pad is pasted on the base plate, and the buffer pad Paste the sample pad on the other side, and then paste the enzyme pad, detection film, and water-absorbing pad in turn on the sample pad; after pasting in sequence, cover the cover plate to obtain the quick test card; Holes and viewing windows, the sample loading holes are facing the buffer pad, and the viewing windows are respectively facing the detection membrane; the upper surface of the detection membrane is coated side by side with organophosphate and carbamate pesticide protein conjugates and antibiotic enzymes Antibody IgG forms the detection line and qual...

Embodiment 2

[0075] Embodiment 2 The structure of the quick test card of the present invention

[0076] 1. As attached figure 1 As shown, the quick test card of the present invention includes a base plate 1 and a cover plate 9 .

[0077] The bottom plate 1 includes a buffer pad 2 , and two sets of sample pads 3 , enzyme pads 4 , detection membrane 5 and water-absorbing pads 8 .

[0078] Wherein, the buffer pad 2 is a whole, spanning two sample pads 3, and the two groups of sample pads 3, enzyme pads 4, detection membrane 5 and water-absorbent pad 8 are pasted on the base plate 1 in sequence; The upper surface of 5 is coated side by side with organophosphate and carbamate pesticide protein conjugates (detection line 6) and anti-biological enzyme antibody IgG (quality control line 7).

[0079] The cover plate 9 includes a sample injection hole 10 and a viewing window 11 .

[0080] 2. The functions and preparation methods of each part of the above-mentioned quick test card of the presen...

Embodiment 3

[0090] Embodiment 3 Application of the quick test card of the present invention

[0091] 1. The enzyme pad 4 in the quick test card of this embodiment is the cholinesterase labeled with the enzyme substrate, which is the main point of the quick test card of this embodiment.

[0092] 2. Utilize this quick test card, according to the detection method provided by the present invention, the minimum detection limits of organophosphorus and carbamate pesticides in several common tea leaves are as shown in table 1 below:

[0093] Table 1 The minimum detection limit (mg / kg) of the cholinesterase immunochromatographic quick test card for several common pesticides in tea

[0094]

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Abstract

The invention discloses a quick detecting card for immunochromatography of organophosphorus and carbamate pesticide multiresidue cholinesterase.The quick detecting card comprises a bottom board and a covering board.A plurality sets of buffering pads are arranged on the bottom board, a plurality of sample pads are crossed on each buffering pad, and enzyme pads, detection films and water absorbing pads are sequentially arranged and pasted in the mode of being close to the sample pads.A plurality of feeding holes and a plurality of windows are formed in the covering board.The upper surfaces of the detection films are wrapped by organophosphorus and carbamate pesticide protein conjugate and anti-bio-enzyme antibodies IgG in parallel.The quick detecting card is rapid and easy to operate; various kinds of organophosphorus and carbamate pesticide in samples can be rapidly detected at the same time, the sensitivity is 0.008 mg / kg-0.987 mg / kg, the result can be observed in 3 minutes to 5 minutes, and the quick detecting card is suitable for quickly detecting organophosphorus and carbamate pesticides in vegetables, fruits and environment water samples.

Description

technical field [0001] The invention belongs to the technical field of pesticide detection. More specifically, it relates to a quick test card for multi-residue cholinesterase immunochromatography of organophosphorus and carbamate pesticides. Background technique [0002] Pesticides are important agricultural production materials used to prevent and control pests in agricultural production, and are widely used in the production of fruits, vegetables and grains. Pesticides play a very important role in ensuring the harvest of agricultural and sideline products. However, in recent years, the abuse of pesticides has also brought a huge threat to the environment and human health. More and more countries are gradually attaching great importance to the abuse of pesticides. Due to the wide variety of pesticides and the common phenomenon of compound use, it is necessary to establish a multi-residue detection method that can detect multiple objects at the same time. Instrumental ...

Claims

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Application Information

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IPC IPC(8): G01N33/58G01N33/558
CPCG01N33/558G01N33/581
Inventor 杨金易曾道平
Owner 广州万联生物科技有限公司
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