Primer and method for detecting hypertension-susceptibility-related SNP site
A susceptibility and high blood pressure technology, applied in the direction of biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problem of undiscovered high blood pressure susceptibility risk detection, and achieve high accuracy and high detection method Simple and specific effect
Inactive Publication Date: 2016-08-03
CHENGDU ZHONGCHUANG QINGKE MEDICAL LAB CO LTD
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Problems solved by technology
But so far, no closely related susceptibility genes and primers have been found for the detection of hypertension susceptibility risk.
Method used
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Embodiment 1
[0037] 1. Collection of samples and extraction of whole genome DNA
[0038] Randomly collect 3.0 mL of peripheral blood from healthy people with EDTA-K2 anticoagulant tubes (three subjects are randomly selected as A, B and C), and use Tiangen Blood Genome Extraction Kit to extract the whole genome DNA of the subjects. The operation process According to the instructions of the kit, the concentration and purity of the obtained whole-genome DNA were detected by an ultra-trace protein and nucleic acid analyzer (BioDropμLite), and the original data was recorded. spare.
[0039] 2. PCR amplification, gel recovery, purification and sequencing of the target gene
[0040] Use the primers of FIGN gene rs16849225 site, ENPEP gene rs6825911 site primers, NPR3 gene rs1173766 site primers and TBX3 gene rs35444 site primers to perform PCR amplification on the extracted DNA respectively. As shown in Table 1;
[0041] Table 1 PCR reaction system
[0042] Reagent
Volume (μL)
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The invention provides a primer for detecting a hypertension-susceptibility-related SNP site .The primer includes an FIGN gene rs16849225 site primer, an ENPEP gene rs6825911 site primer, an NPR3 gene rs1173766 site primer and a TBX3 gene rs35444 site primer .A method for detecting the hypertension-susceptibility-related SNP site with the primer includes the following steps that 1, samples are collected, and genome DNA of the samples is extracted; 2, the genome DNA is subjected to PCR amplification through the four primers respectively, and the amplification products are subjected to gel recovery; 3, the gel recovery products are subjected to concentration measurement and PCR amplification and purified; 4, the purified products are sequenced, and the sequenced results of the SNP site are analyzed .The primer is good in specificity, high in sensitivity and good in accuracy, the detection method is simple, and the hypertension suffering probability of a detected patient can be forecasted.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a primer and a detection method for detecting SNP sites related to susceptibility to hypertension. Background technique [0002] Hypertension refers to a clinical syndrome mainly manifested by elevated systemic arterial pressure. Long-term hypertension is an important cause of death from cardiovascular diseases. The incidence of hypertension is the result of the joint action of genetic factors and environmental factors. In the past, candidate gene association studies were used to conduct gene association studies on renal sodium transport and renin-angiotensin-aldosterone system (RAS)-related protein-coding genes based on the results of existing studies on the mechanism of hypertension. Although a large number of candidate gene association studies have identified some suspected causative genes, these findings are often not reproducible. With the development of molecular b...
Claims
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Login to View More IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 刘月张蓉李江林刘宇
Owner CHENGDU ZHONGCHUANG QINGKE MEDICAL LAB CO LTD