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A method for rapid propagation of Striga in tissue culture

A technology of tissue culture and Striga, which is applied in the field of plant tissue culture, can solve the problems of low germination rate, scarce wild Striga resources, and small Striga seeds, so as to shorten the seedling time, save planting costs, and improve reproduction effect of ability

Active Publication Date: 2017-10-24
GUANGDONG KING ZUO AGRI SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Striga is suitable for growing in low-altitude barren hills, grasslands, fields, valleys, cultivated land, etc. It is mainly distributed in the provinces and regions from southwest to southeast of my country, but wild Striga resources in each area are scarce, which cannot meet the needs of the current stage. This is mainly due to the tiny seeds of Striga, low germination rate, and slow growth, and Striga is a semi-parasitic plant, which has strict requirements on the growth environment, making wild Striga resources very scarce

Method used

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  • A method for rapid propagation of Striga in tissue culture
  • A method for rapid propagation of Striga in tissue culture
  • A method for rapid propagation of Striga in tissue culture

Examples

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Embodiment 1

[0027] A tissue culture rapid propagation method of Striga is characterized in that, it is processed by the following steps:

[0028] 1) picking wild Striga plants, cutting into sections so that each section contains an axillary bud;

[0029] 2) put the sectioned plants containing axillary buds in step 1) into 0.1% mercuric chloride solution for disinfection for 8-10 minutes, and then wash them three times with sterile water, each time for 2-3 minutes;

[0030] 3) Inoculate the section plants containing axillary buds in step 2) into the induction medium for cultivation on the ultra-clean workbench, wherein the induction medium is composed of the following components: 1L MS medium is added with 3mg 6-BA and 0.1mg IBA, then use NaOH to adjust the pH of the induction medium to 5.5-6.2, and cultivate it under the artificial light intensity of 1900-2100Lx and the temperature of 25-35°C until adventitious buds grow, and then transfer the adventitious buds to Carry out multiple subc...

Embodiment 2

[0035] A tissue culture rapid propagation method of Striga is characterized in that, it is processed by the following steps:

[0036] 1) picking wild Striga plants, cutting them into sections so that each section contains a terminal bud;

[0037] 2) put the sectioned plant containing terminal buds in step 1) into 0.1% mercuric chloride solution for disinfection for 8-10 minutes, and then wash with sterile water three times, each time for 2-3 minutes;

[0038] 3) On the ultra-clean workbench, inoculate the sectioned plants containing terminal buds in step 2) into the induction medium for cultivation, wherein the induction medium is composed of the following components: 3mg 6-BA is added to 1L MS medium and 0.1mg IBA, then use NaOH to adjust the pH of the induction medium to 5.5-6.2, and cultivate it under the artificial light intensity of 1900-2100Lx and the temperature of 25-35°C until adventitious buds grow, and then transfer the adventitious buds Carry out multiple inductio...

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Abstract

The invention provides a tissue culture and rapid propagation method of striga asiatica. The method mainly comprises: picking axillary bud stem fragments of wild striga asiatica as explants, putting the sterilized explants in an induction medium for culture until adventitious buds are generated, putting the adventitious buds in a new induction medium for subculture to induce the adventitious buds for rapid proliferation, and putting an individual plant in a rooting medium for culture to induce roots of the adventitious buds and obtaining a lot of reproduction plants. According to the tissue culture and rapid propagation method of striga asiatica, an axillary bud can produce 10-20 new buds in two months, thereby improving the reproductive capacity of striga asiatica, shortening the seedling production time, and saving the plant cost. Therefore, the technical support is provided for industrialized seedling production of striga asiatica.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and specifically relates to a method for rapid propagation of striga by inducing terminal buds or axillary buds of striga to produce adventitious buds. Background technique [0002] Striga, which belongs to Scrophulariaceae Striga, is a commonly used medicinal plant. It has great medicinal value in treating children with clearing liver heat and eliminating malnutrition. It also has certain medicinal value in anti-inflammatory, anti-inflammatory and other aspects. Striga is suitable for growing in low-altitude barren hills, grasslands, fields, valleys, cultivated land, etc. It is mainly distributed in the provinces and regions from southwest to southeast of my country, but the resources of wild striga in each area are scarce, which cannot meet the needs of the current stage. This is mainly due to the tiny seeds of Striga, low germination rate, and slow growth, and Striga is a semi-parasitic pla...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 蔡时可王继华罗焕明
Owner GUANGDONG KING ZUO AGRI SCI & TECH CO LTD
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