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Application of cuprous oxide nanoparticles to preparation of medicine for treating renal cancer diseases

A technology of cuprous oxide and nanoparticles, applied in the field of medicine, can solve problems such as unclear mechanism

Inactive Publication Date: 2016-08-10
SHANGHAI CHANGHAI HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that copper metabolism in tumor tissue is abnormally active, but the mechanism of pharmacodynamics and signaling pathways of cuprous ions carried by cuprous oxide nanoparticles (CONPs) on renal cancer tissue is still unclear

Method used

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  • Application of cuprous oxide nanoparticles to preparation of medicine for treating renal cancer diseases
  • Application of cuprous oxide nanoparticles to preparation of medicine for treating renal cancer diseases
  • Application of cuprous oxide nanoparticles to preparation of medicine for treating renal cancer diseases

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Experimental program
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Embodiment Construction

[0029] The specific implementation manners provided by the present invention will be described in detail below.

[0030] 1 Experimental materials

[0031] 786-O cells were grown in RPMI1640 medium (Gibco / BRL Company) with 10% fetal bovine serum (FBS), A498 cells were grown in 10% FBS MEM medium (Gibco / BRL Company), at 37°C, 5% Continuous cultivation in the incubator. The CCK-8 kit was purchased from Shanghai Dongren Chemical Technology Co., Ltd., and the AnnexinV / FITC apoptosis kit and cell cycle kit were purchased from Hangzhou Lianke Biotechnology Co., Ltd. Transwell cells were purchased from Corning Incorporated, USA.

[0032] 2 methods

[0033] 2.1, cell proliferation experiment

[0034] In a 96-well plate, add 200 μl of cell suspension to each well, 3000 cells per well, incubate overnight, discard the medium, add CONPs according to the concentration gradient, except for the control group, the concentration gradient of the experimental group is set to 1.25, 2.5, 5, 10...

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Abstract

The invention relates to the application of cuprous oxide nanoparticles in the preparation of drugs for treating kidney cancer, and the kidney cancer is clear cell type kidney cancer, granular cell type kidney cancer, mixed cell type kidney cancer and undifferentiated cell type kidney cancer. The invention also provides a medicine for treating kidney cancer. The invention adopts the sequencing of the expression profile after the action of the kidney cancer drug, the in vivo experiment of the drug acting on the cell line, and the in vitro experiment of the subcutaneous tumor bearing in nude mice. Further explain the therapeutic role of CONPs in RCC and related signaling pathways, and lay a theoretical foundation for exploring new treatments for metastatic RCC.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of cuprous oxide nanoparticles in the preparation of medicines for treating kidney cancer. Background technique [0002] Renal cancer accounts for 2-3% of all malignant tumors. Although some patients can be cured by surgery, 20-30% of patients have developed distant metastasis when they are diagnosed, and 30-40% of patients have postoperative recurrence and metastasis. Kidney cancer is not sensitive to radiotherapy and chemotherapy. Tumor tissue has special high permeability and retention, and nano-sized carriers can pass through the gaps in the tumor vascular endothelial system, carry drugs into and accumulate in tumor tissues; secondly, nanocarriers can achieve intelligent drug delivery in vivo through functional modification. It has the functions of targeted positioning and drug release control, and at the same time reduces the toxic and side effects of drug...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K33/34A61P35/00
CPCA61K9/5107A61K33/34
Inventor 孙颖浩杨启维杨庆崔心刚王野
Owner SHANGHAI CHANGHAI HOSPITAL
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