In-vitro maturation culture solution for small follicle oocytes and application thereof

Abstract

The invention relates to cell culture, and particularly discloses an in-vitro maturation culture solution for small follicle oocytes .The culture solution contains 100 nM E2 and 100ng / mL CNP / BNP .The invention further provides application of the in-vitro maturation culture solution in promoting small follicle oocyte in-vitro maturation or inhibiting small follicle oocyte meiosis and a culture method for promoting oocyte in-vitro maturation .The method comprises the steps that animal oocytes are collected and classified according to the diameter; the small follicle oocytes are placed in the in-vitro maturation culture solution for pre-maturation culture for 20 h and then cultured in a maturation solution for 46+ / -2 h; the large follicle oocytes are directly cultured in the maturation solution for 46+ / -2 h .Two stages of in-vitro maturation culture methods are adopted, the CNP / BNP and E2 are applied for pre-maturation treatment of the small follicle oocytes, synchronization of nuclear maturation and cytoplasm maturation is promoted, the in-vitro viability is improved, and the in-vitro maturation culture solution can become novel meiosis inhibiting medicine to be applied and popularized in animal husbandry yield .

Description

technical field [0001] The present invention relates to cell culture, in particular to the in vitro maturation culture solution of small follicle oocytes. Background technique [0002] In livestock breeding, in vitro maturation of unstimulated ovary-derived oocytes is widely used to produce animal embryos. Therefore, in vitro oocyte maturation is an important platform technology for breeding, cloning and transgenic animal production. [0003] At present, the number of high-quality breeding males is small, the breeding speed is slow, and the production performance is backward, which are the key factors restricting the development of animal husbandry in my country. The cell number of in vitro fertilized embryos is significantly lower than that of in vivo embryos, which reflects that the in vitro culture system of oocytes is not ideal. The quality of in vitro fertilized embryos is an important factor affecting the pregnancy rate of embryo transfer and the survival rate of offs...

AI Technical Summary

Problems solved by technology

[0005] However, in simulating the in vivo environment, a two-stage maturation method of oocytes in vitro was developed, and the research results showed that meiosis inhibitors did not improve the ability of oocytes to develop in vitro, and even had adverse effects

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