Cell mixture and preparation method and application thereof

Abstract

The invention belongs to the technical field of laboratory animals, and relates to a cell mixture and a preparation method and application thereof, especially, to an application of a mixture of adipose-derived stem cells (ADSCs) and immortalized human umbilical vein endothelial cells to preparation of a preparation for interfering and establishing a vascular malformation animal model. The mixture of adipose-derived stem cells (ADSCs) and immortalized human umbilical vein endothelial cells is used for interfering and establishing a vascular malformation animal model. The result shows that the vascular malformation animal model can be obtained at low cost, in a short period, and with high efficiency; and the established vascular malformation animal model of a nude mouse is obviously visible in vascular malformation and makes a significant practical sense in researching pathogenesis of vascular malformation and screening of clinical medicine treatment.

Description

technical field [0001] The invention belongs to the technical field of experimental animals, and relates to a cell mixture and its preparation method and application, in particular to the application of the mixture of adipose stem cells (ADSCs) and immortalized umbilical vein endothelial cells in the preparation of preparations for intervention and establishment of vascular malformation animal models. Background technique [0002] The prior art discloses that vascular malformation is a kind of vascular hyperplasia that is prone to occur in various parts of the body of infants, and about 71% of vascular malformations are prone to occur in the head, face and neck, which often cause serious facial deformities and affect their physical and mental health. healthy growth. The main pathology of said vascular malformation is excessive proliferation of vascular endothelial cells and formation of abnormal vascular lumen. Clinically, vascular malformations can be roughly divided into ...

AI Technical Summary

Problems solved by technology

In recent years, the biological characteristics, clinical and pathological classification and treatment of vascular malformations have been the research hotspots in this field. Especially with the development of molecular biology and stem cell biology, scholars at home and abroad have successively reported on the pathogenesis and treatment of vascular malformations. related mechanism; however, the lack of suitable animal models of vascular malformations seriously restricts the research on vascular malformations, therefore, the establishment of an experimental animal model of vascular malformations has practical significance for the research of the pathogenesis of vascular malformations and the screening of clinical drug treatments

[0003] Related hemangioma models have been reported in the literature, but the establishment of vascular malformation models is rarely reported

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