Cell mixture and preparation method and application thereof

A mixture and cell technology, applied in the field of experimental animals, can solve the problems of lack of animal models of vascular malformations, few reports of vascular malformation models, restricting research on vascular malformations, etc., and achieve low-cost results.

Inactive Publication Date: 2016-08-10
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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Problems solved by technology

In recent years, the biological characteristics, clinical and pathological classification and treatment of vascular malformations have been the research hotspots in this field. Especially with the development of molecular biology and stem cell biology, scholars at home and abroad have successively reported on the pathogenesis and treatment of vascular malformations. related mechanism; however, the lack of suitable animal models of vascul

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  • Cell mixture and preparation method and application thereof
  • Cell mixture and preparation method and application thereof
  • Cell mixture and preparation method and application thereof

Examples

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Embodiment 1

[0037] In the present embodiment, described enzymatic enzyme is dispase, purchased from sigma company (dispase is also called dispase, neutral protease)

[0038] In this example, a mixture of adipose stem cells (ADSCs) and immortalized human umbilical vein endothelial cells (HUVEC) was used to make a seed cell preparation for effective intervention and establishment of animal models of vascular malformation.

[0039] First, adipose stem cells (ADSCs) were obtained by the following method;

[0040] The isolated sterile adipose tissue, chopped into 1mm 3 , placed in DMEM medium for washing, the volume of the DMEM medium is 5-10 times the volume of adipose tissue; centrifuge and discard the supernatant;

[0041] Digest the adipose tissue fragments for 45-90 minutes with a digestive enzyme solution with a mass-volume ratio of 5-20 times the tissue volume of 0.05-0.3%; the digestive enzyme is enzyme enzyme, collagenase NB4 or a mixture of the two The digestive enzyme can use the ...

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Abstract

The invention belongs to the technical field of laboratory animals, and relates to a cell mixture and a preparation method and application thereof, especially, to an application of a mixture of adipose-derived stem cells (ADSCs) and immortalized human umbilical vein endothelial cells to preparation of a preparation for interfering and establishing a vascular malformation animal model. The mixture of adipose-derived stem cells (ADSCs) and immortalized human umbilical vein endothelial cells is used for interfering and establishing a vascular malformation animal model. The result shows that the vascular malformation animal model can be obtained at low cost, in a short period, and with high efficiency; and the established vascular malformation animal model of a nude mouse is obviously visible in vascular malformation and makes a significant practical sense in researching pathogenesis of vascular malformation and screening of clinical medicine treatment.

Description

technical field [0001] The invention belongs to the technical field of experimental animals, and relates to a cell mixture and its preparation method and application, in particular to the application of the mixture of adipose stem cells (ADSCs) and immortalized umbilical vein endothelial cells in the preparation of preparations for intervention and establishment of vascular malformation animal models. Background technique [0002] The prior art discloses that vascular malformation is a kind of vascular hyperplasia that is prone to occur in various parts of the body of infants, and about 71% of vascular malformations are prone to occur in the head, face and neck, which often cause serious facial deformities and affect their physical and mental health. healthy growth. The main pathology of said vascular malformation is excessive proliferation of vascular endothelial cells and formation of abnormal vascular lumen. Clinically, vascular malformations can be roughly divided into ...

Claims

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Application Information

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IPC IPC(8): C12N5/0775C12N5/071A01K67/027
Inventor 陆信武袁福康秦金保彭智猷叶开创杨心蕊黄丽佳蒋米尔
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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