Inositol detection method

Abstract

An inositol detection method comprises the following steps: mixing a blood plasma sample and anhydrous ethanol, adding the mixture into a sample concentration glass tube, putting the glass tube into a Biotage TurboVapLV multi-sample automatic concentration instrument, adjusting a nitrogen generator to generate nitrogen of 60 Psi pressure, blow-drying the sample with nitrogen after 10 min, adding 5mL of a silylating reagent, conducting a derivative reaction in a water bath of 70 DEG C for 1 h, taking out and cooling to room temperature, adding 5mL of n-hexane and 10mL of saturated NaCl, carrying out uniform vortex mixing, transferring into a 50mL centrifuge tube, centrifuging at 6000 rmp for 5 min, fetching a supernatant and blow-drying with nitrogen, and making constant volume of n-hexane to 1mL. According to the invention, human blood plasma samples and inositol standard substances undergo derivatization, and the derivatization technology is optimized. After purification extraction, a gas chromatography-mass spectrometer is adopted for analysis. Thus, detection results are accurate, and sample volume is the minimum. The method of the invention can be applied in detection of blood plasma samples with neural tube birth defects.

Description

technical field [0001] The invention belongs to the field of applied basic medical research, and relates to the application of gas chromatography-mass spectrometry technology in detecting plasma myo-inositol level. Background technique [0002] Inositol, also known as cyclohexyl alcohol, is a colorless, water-soluble, sweet, acid, alkali and heat-resistant polyhydric hydrocarbon derivative of cyclohexane. Myo-inositol (MI) is the most widely distributed in nature. In the human body, in addition to food sources, inositol can also be synthesized in brain, testis and kidney tissues by 6-phosphate glucose through the action of key enzymes in inositol synthesis. The majority of inositol is found in the brain, cardiac and skeletal muscles. The content of inositol in normal human brain tissue is 200 times that in blood. In cells, inositol forms inositol derivatives with different phosphate groups through phosphorylation and dephosphorylation reactions, participates in different ...

AI Technical Summary

Problems solved by technology

In the national standard method for the determination of inositol in infant foods and dairy products, the substance to be tested undergoes a derivatization reaction in the presence of water, and the silylating reagent directly reacts with water, which results in a derivatization efficiency for plasma samples with complex matrices. low, affecting the test results

[0004] As mentioned above, although the GC / MS method is currently recognized as a method that can accurately detect inositol, for biological samples with complex matrices such as plasma, there is still a lack of accurate, rapid and stable accurate methods

At the same time, due to the particularity of plasma samples, the sample volume required for testing, the storage time of samples and other factors can affect the clinical applicability of the testing method.

The present invention is further improved on the basis of the established GC / MS method for detecting mixed plasma inositol, so as to be better applied to the detection of clinical samples, and no related reports have been seen so far

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