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A kind of Pseudomonas betae producing keratinase and its application

A technology of pseudomonas and keratinase, applied in the field of microorganisms, can solve the problems of difficult direct utilization, energy and environmental pollution, difficult biodegradation, etc., and achieve the effect of considerable application prospects.

Active Publication Date: 2019-05-07
GUANGDONG WENS DAHUANONG BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because its main component, keratin, is a hard protein with strong resistance and refractory biodegradation, it is difficult to use directly, and traditional physical and chemical processing methods (high temperature and pressure, acid-base treatment, etc.) not only consume a lot of energy but also cause serious environmental pollution

Method used

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  • A kind of Pseudomonas betae producing keratinase and its application
  • A kind of Pseudomonas betae producing keratinase and its application
  • A kind of Pseudomonas betae producing keratinase and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: Isolation and cultivation of keratinase-producing bacteria

[0026] Place the collected soil samples in sterile distilled water and mix them evenly, let stand to get the supernatant, and carry out gradient dilution (dilution to 10 -6 、10 -7 、10 -8 ), were respectively spread on the milk agar plate medium, and cultured upside down in a 37°C incubator for 24h. Select a single colony that can produce a transparent degradation circle, inoculate it in an inorganic liquid medium containing a feather of about 0.5 g, and cultivate it at 37° C. with shaking at 180 rpm for 72 hours. The culture fluid of strains with obvious feather degradation was selected and inoculated into a new inorganic liquid medium for re-screening culture.

[0027] Inoculate the strains obtained by re-screening into a 250mL Erlenmeyer flask containing a complete feather and 50mL of inorganic liquid medium, culture at 35°C and 200rpm with shaking for 48h, observe the degradation of feather...

Embodiment 2

[0030] Example 2: Identification of keratinase-producing bacteria

Embodiment 2A

[0031] Embodiment 2A) morphological characteristics

[0032] The isolated and purified target strain D1 was streaked and inoculated on milk agar plate medium, and cultured at 37°C for 24 hours. Visually inspect the colonies for shape, color, surface texture, and colony edges. The morphology of bacteria was observed by optical microscope and transmission electron microscope. Refer to the "Common Bacteria System Identification Manual" to identify the physiological and biochemical characteristics of the strains and make a preliminary classification.

[0033] The shape of the target colony under the transmission electron microscope is as follows: figure 1 shown. The colony of the target strain D1 was cultured on the milk agar plate medium for 24 hours to produce a transparent degradation circle, the colony was a yellow single colony with smooth edges; Gram staining was negative; short-chain rods, no spores, no capsule, and flagella .

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Abstract

The invention provides a keratinase-producing Pseudomonas beta, and its preservation number is CGMCC No. 12183. The present invention also provides the application of the keratinase-producing Pseudomonas beta in degrading keratin. After 24 hours of fermentation and cultivation, the degradation rate to feathers can be as high as 60%, and its keratinase activity reaches 35.6U / mL. Pseudomonas beta keratinase has considerable application prospects in the field of degradation and reuse of livestock and poultry wastes containing keratin.

Description

technical field [0001] The invention relates to a microbial technology, in particular to a keratinase-producing Pseudomonas beta and application thereof. Background technique [0002] Feather is an important protein resource, and its protein content is as high as 85-90%. However, because its main component, keratin, is a hard protein with strong resistance and difficult biodegradation, it is difficult to use directly, and traditional physical and chemical processing methods (high temperature and high pressure, acid-base treatment, etc.) not only consume a lot of energy but also cause Serious environmental pollution problem. At present, more than one million tons of feather waste are produced in my country's aquaculture industry every year. With the continuous development of my country's aquaculture industry, the number is still growing. It is an inevitable choice to realize high-value transformation and recycling of waste resources. Research on the transformation and appli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/38
CPCC12N1/205C12R2001/38
Inventor 黄妙容钟楚红刘传高任广彩陈瑞爱叶俊贤
Owner GUANGDONG WENS DAHUANONG BIOTECH