Primer for auxiliary identification of soybean anti-mosaic virus SC7 and identification method thereof

A technology for auxiliary identification and detection methods, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the problems of low breeding efficiency and achieve improved pertinence, high economic benefits, and improved efficiency Effect

Pending Publication Date: 2016-08-17
FUYANG CITY ACADEMY OF AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0002] In conventional soybean breeding, phenotypic selection is easily restricted by factors such as seasons and production safety environments, resulting in low breeding efficiency. Modern agriculture urgently needs to inject biotechnology means, supplemented by molecular markers. Ideal new soybean variety

Method used

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  • Primer for auxiliary identification of soybean anti-mosaic virus SC7 and identification method thereof
  • Primer for auxiliary identification of soybean anti-mosaic virus SC7 and identification method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0024] The above primers were used to detect soybean mosaic virus SC7 resistance.

[0025] S1, 24 soybean resource materials (homozygous varieties or strains) were randomly collected from the regional test of Fuyang Soybean Comprehensive Experimental Station of the modern agricultural industrial technology system, and the DNA of each soybean was extracted by the SDS method, and the primers were used to carry out PCR amplification, the PCR amplification reaction system is 20 μL, including 2.0 μL of 10×PCRBuffer (containing 15 mM Mg2+), 0.2 μL of dNTP with a final concentration of 10 mM, 0.2 μL of Tag enzyme with a concentration of 2.5 U / μl, and 0.08 μL of the front primer. μl, the back primer 0.08 μl, ddH 2 O12.44 μL, 5 μL template DNA with a concentration of 20 ng / μL; the PCR reaction program was pre-denaturation at 94°C for 5 min; denaturation at 94°C for 30 sec, annealing at 55°C for 30 sec, extension at 72°C for 1 min, and 35 cycles, and then extended at 72°C for 8 min. St...

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Abstract

The present invention discloses a primer for auxiliary identification of a soybean anti-mosaic virus SC7 and an identification method thereof. A BARCSOYSSR_02_0631 molecular marked special primer is used to conduct early progeny selection identification on related sites of a soybean strain anti-mosaic virus SC7. The method not only avoids spread of mosaic virus in the traditional inoculation identification method, but also reduces the subsequent workload of breeding and breeding cycles, so as to quickly screen a resistant high-yield soybean breeding material. The method has high economic efficiency, can be applied to the resistance breeding of soybean progeny, can improve breeding efficiency and resistance level of soybean mosaic virus, and improve the pertinency of breeding purpose.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a primer for assisting identification of soybean anti-mosaic virus SC7 and a detection method thereof. Background technique [0002] In conventional soybean breeding, phenotypic selection is easily restricted by factors such as seasons and production safety environments, resulting in low breeding efficiency. Modern agriculture urgently needs to inject biotechnology means, supplemented by molecular markers. An ideal new soybean variety was bred in an efficient manner. With the rapid development of bioinformatics, molecular genetics and genomics, the application of molecular marker-assisted selection breeding will be more extensive. PCR-based molecular markers (such as SSR) have the advantages of co-dominance, high polymorphism, good repeatability, and easy operation, and are widely accepted by genetic breeders. [0003] Many experts and scholars at home and abroad have carrie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/686C12Q1/6895C12Q2600/13
Inventor 王传之孙云飞刘武艺李扬眉王敏王梓钰李智民李素梅冯邦杰刘新亮马慧慧白鹏陈乃珍闫振张婧霖李铭
Owner FUYANG CITY ACADEMY OF AGRI SCI
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