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Application of steroids in preparing drugs for treating rheumatoid arthritis

A technology of steroids and compounds, applied in the field of phytochemistry, can solve problems such as unreported

Active Publication Date: 2016-08-31
HAINAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been reports on the steroidal compounds isolated from Melaleuca japonica, but there are no reports on the application of these compounds in inhibiting the proliferation of rheumatoid arthritis synoviocytes

Method used

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  • Application of steroids in preparing drugs for treating rheumatoid arthritis
  • Application of steroids in preparing drugs for treating rheumatoid arthritis
  • Application of steroids in preparing drugs for treating rheumatoid arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1: Preparation of steroidal compounds.

[0040] 10Kg of the dried stems of S. mellifera were routinely extracted with 75% v / v ethanol 3 times for 7 days each time to obtain an extract, which was concentrated under reduced pressure into a paste to obtain 1200 g of S. mellifera extract. The extract was diluted into a suspension with distilled water (3 L), extracted with petroleum ether (3 L x 3 times) and ethyl acetate (3 L x 3 times) successively, and the ethyl acetate extract was concentrated into an extract (about 250 g), Carry out silica gel column chromatography, use petroleum ether-ethyl acetate mixed solvent (100:0-0:100, V / V) and ethyl acetate-methanol (100:0-0:100, V / V) according to polarity Perform silica gel column chromatography incrementally, and collect fractions of about 400 mL each time. Through TLC detection, the combined similar fractions were divided into 8 components, namely Fr.1-8. Fr.2 Use 200-300 mesh silica gel on the column, use ethyl...

Embodiment 2

[0045] Embodiment 2: pharmacological activity experiment

[0046] Experimental Materials

[0047] 1. Experimental materials

[0048] 1.1 Cells: Rat Synovial Cells (RSC)

[0049] 1.2 Cell culture medium: DMEM base solution (Hyclone) containing 10% calf serum (GIBCO), 100 U / ml penicillin (GIBCO), and 100 U / ml streptomycin (GIBCO).

[0050] 1.3 Reagents: MTT, sigma; DMSO, Sinopharm

[0051] 3. Experimental method

[0052] 2.1 Grouping

[0053] Divided into 5 groups, as follows:

[0054] Negative control group: C, containing cells, without administration;

[0055] Positive control group: methotrexate, 250 μg / ml);

[0056] Administration group: 1.0μg / ml, 10μg / ml, 50μg / ml, 100μg / ml;

[0057] 2.2 Test the growth inhibitory effect of the above-mentioned 3 compounds obtained from Melaleuca officinalis on RSC cells.

[0058] Detection by MTT colorimetric method: use the traditional method of 96-well plate, inoculate cells in 96-well plate before administration, 1x10 4 cells / we...

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Abstract

The invention discloses an application of steroids of extract of Fissistigma oldhamii(Hemsl.) Merr. in preparing drugs for treating rheumatoid arthritis. The structural formula of the steroids is as shown in the specification; wherein the stub symbol in the structural formula can be double bonds or single bond; R1, R2 and R3 are respectively -OH or -CH3 or -CH2H5. The activity of the steroids in suppressing synoviocyte proliferation of rheumatoid arthritis is discovered for the first time, and the steroids can be applied to preparation of the drugs for treating rheumatoid arthritis.

Description

technical field [0001] The invention relates to the technical field of phytochemistry, in particular to the application of a steroid compound in the preparation of drugs for treating rheumatoid arthritis. Background technique [0002] Fissistigma oldhamii (Hemsl.) Merr., also known as Fissistigma oldhamii (Hemsl.) Merr., is a plant of the genus Fissistigma oldhamii (Annonaceae) in the tropical and subtropical Annonaceae family. The plant is mainly distributed in southern my country and Vietnam. The plant can be used as medicine. The stems and leaves can cure fractures and edema, and the whole plant can treat rheumatic bone pain and numbness of hands and feet. There have been reports on the steroidal compounds isolated from Melaleuca japonica, but there is no report on the application of these compounds in inhibiting the proliferation of rheumatoid arthritis synoviocytes. Contents of the invention [0003] The technical problem to be solved by the present invention is to p...

Claims

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Application Information

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IPC IPC(8): A61K31/575A61P29/00A61P19/02
CPCA61K31/575
Inventor 陈光英周学明韩长日宋小平徐伟郑彩娟
Owner HAINAN NORMAL UNIVERSITY
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