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Method for liquid chromatography-mass spectrometry tandem detection of content of stachydrine hydrochloride in Fukangning tablets

A technology of stachydine hydrochloride and Fukangning tablets is applied in the field of chemical detection to achieve the effect of rapid detection and perfect quality standards

Inactive Publication Date: 2016-08-31
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After reviewing the literature, there is no report on the method for determining the content of stachydrine hydrochloride in Fukangning Tablets

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1) Take 10 tablets of this product, remove the coating, accurately weigh, grind finely, take about 2g, accurately weigh, put it in a conical flask, accurately add 20ml of 80% ethanol, seal it tightly, weigh it, and extract it by ultrasonic for 30min , let it cool, weighed again, supplemented the lost weight with 80% ethanol, shook well, passed through the C18 solid-phase extraction column activated with 3ml ethanol and 3ml water respectively at a flow rate of 1ml / min, rinsed with 5ml water, Discard, then elute with 5ml of ethanol to collect the eluate, blow the eluate to nearly dry under nitrogen at 40°C, add 1ml of ethanol to dissolve it, filter it with a 0.22μm organic filter membrane, and take the filtrate to obtain the test solution ;

[0018] 2) Get the motherwort negative sample prepared according to the prescription ratio and process, and prepare a negative control solution according to the preparation method of the test solution;

[0019] 3) Accurately weigh 17...

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Abstract

The invention provides a method for liquid chromatography-mass spectrometry tandem detection of the content of stachydrine hydrochloride in Fukangning tablets, and aims to provide the method used for liquid chromatography-mass spectrometry tandem detection of the content of stachydrine hydrochloride in the Fukangning tablets and having the advantages of high sensitivity and accurate qualitation and quantitation; the method comprises the steps: 1) preparing a test sample solution; 2) preparing a negative reference solution; 3) preparing a reference solution; and 4) respectively weighing the test sample solution, the reference solution and the negative reference solution, loading in an Agilent1295-6550C triple quadrupole liquid chromatography-mass spectrometer, and detecting. The method belongs to the technical field of chemical detection.

Description

technical field [0001] The invention provides a method for measuring the content of stachydrine hydrochloride, specifically, a method for detecting the content of stachydrine hydrochloride in Fukangning tablets by LC / MS series; it belongs to the technical field of chemical detection. Background technique [0002] The prescription of Fukangning Tablets is composed of eight traditional Chinese medicines such as white peony root, motherwort, Cyperus cyperi, etc. It has the effects of nourishing blood and regulating qi, promoting blood circulation and regulating menstruation, and it mainly treats irregular menstruation caused by blood deficiency and stagnation of qi. Motherwort in the prescription is a good medicine for gynecology in traditional Chinese medicine. It belongs to the fresh or dry aerial part of Leonurus iaponicus Houtt., a labiatae plant. For irregular menstruation, amenorrhea dysmenorrhea, endless lochia, edema, oliguria, sore and swollen toxin. [0003] Fukangni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 陈学松陈江涛梁慧敏李亚李澄
Owner WUZHOU INST FOR FOOD & DRUG CONTROL