A method to broaden the genetic variation of the B subgenome of Brassica juncea using black mustard
A genetic variation and genome technology, which is applied in the field of using black mustard to broaden the field of genetic variation of the mustard B subgenome, can solve the problems of long breeding cycle and compatibility obstacles, and achieve the advantages of short breeding cycle, simple process, and broadening of the genetic basis. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0026] 1), the acquisition of triploid:
[0027] Brassica napus A r A r (The strain is 4M245) is the female parent, the buds are peeled and emasculated, and the mustard type rape A is awarded j A j B j B j (The strain is 4N006) pollen, sexual hybridization, bagged and grown for a week, then unpacked, then grown to maturity under natural conditions, and harvested triploid seeds A r A j B j ;
[0028] 2), the acquisition of hexaploid:
[0029] With the triploid seed A harvested in step 1) r A j B j Sow and germinate, place a cotton ball soaked in a 0.01% colchicine solution at the growth point at the 6-leaf stage of the plant, and treat it continuously for three days, adding the same concentration of colchicine solution to the cotton ball 3 times a day, during the treatment. The plastic film covers the top of the plant; remove the cotton and plastic film after treatment, the plant grows under natural conditions, detect its pollen fertility and fruiting at the floweri...
Embodiment 2
[0034] The present embodiment is based on embodiment 1, the Chinese cabbage type rape A in embodiment 1 r A r (The strain is 4M245) replaced by vegetable cabbage A r A r (the strain is 5W245); the Brassica napus A j A j B j B j (The strain is 4N006) replaced with vegetable mustard A j A j B j B j (the strain is 4N008, mustard); and mustard A for vegetables j A j B j B j (The strain is 4N008) is the female parent, the buds are peeled and emasculated, and the vegetable cabbage A is given r A r (Strain 5W245) pollen was sexually crossed.
[0035] In this example: the hybridization of hexaploid and 3 different genotypes of black mustard has a certain degree of matability, and the average seed setting rate is 3.55 grains / silique; the number of somatic chromosomes of the new mustard is detected, and they The number of chromosomes is 36; these new mustard seeds can be self-seed, and the average self-seed rate is 6.69 grains / silique. The leaf color, leaf shape, ballin...
Embodiment 3
[0037] The present embodiment is based on embodiment 1, and the difference with embodiment 1 is: with mustard type rape A j A j B j B j (The strain is 4N006) as the female parent, the buds were peeled and emasculated, and the cabbage-type rape A was awarded r A r (Strain 4M245) pollen was sexually crossed.
[0038] In this example: the hybridization of hexaploid and 3 different genotypes of black mustard has a certain degree of matability, and the average seed setting rate is 3.88 grains / silique; the number of somatic chromosomes of the new type of mustard is detected and found that they The number of chromosomes is 36; the self-fertilization of these new mustard plants has good self-fertility, with an average of 6.92 grains per silique. The morphological characteristics of the new mustard leaves produced by crossing the hexaploid with three different genotypes of black mustard have obvious differences in leaf color, leaf shape and other morphological characteristics, and...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More