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Bifidobacterium breve and application thereof in preparing conjugated linoleic acid or conjugated linolenic acid

A technology of Bifidobacterium breve and conjugated linoleic acid, applied in the direction of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve reagent residue, low yield, difficulties in separation and purification of conjugated linolenic acid, etc. question

Active Publication Date: 2016-09-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, among the many plant seeds containing conjugated linolenic acid, only Trichosanthes can be eaten directly, and the oil composition in plant seeds is very complex, so it is very difficult to separate and purify conjugated linolenic acid from plant seed oils
[0006] On the other hand, in the prior art, conjugated linolenic acid can also be produced by alkali-treated linolenic acid isomerization, but its yield is low and there are reagent residues, so the industrial production of conjugated linolenic acid has not yet been realized.

Method used

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  • Bifidobacterium breve and application thereof in preparing conjugated linoleic acid or conjugated linolenic acid
  • Bifidobacterium breve and application thereof in preparing conjugated linoleic acid or conjugated linolenic acid
  • Bifidobacterium breve and application thereof in preparing conjugated linoleic acid or conjugated linolenic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Collection of samples and isolation and identification of bifidobacteria

[0038] Fecal samples of newborns were collected from the Ninth People's Hospital of Wuxi City.

[0039]Take 1g of neonatal feces sample, spread it on mMRS solid medium after serial dilution, culture it at 37°C in an anaerobic environment for 72 hours, observe and record the colony shape, pick and purify the colony, and then put it in MMRS liquid medium Cultivate at 37°C for 48 hours, perform Gram staining on the obtained colonies and record the strain morphology, discard the Gram-negative strains and Gram-positive cocci in the colonies, and select the Gram-positive bacilli, which are analyzed by catalase Afterwards, the catalase-positive strains were discarded, and the catalase-negative strains were kept, and the negative strains were detected by fructose-6-phosphate kinase. The obtained strains were all identified as Bifidobacterium breve by 16S rDNA sequencing, and named as Bifidobac...

Embodiment 2

[0048] Example 2: Bifidobacterium breve C11 (CCFM683) biotransformation of conjugated linoleic acid

[0049] The specific experiment is as follows:

[0050] 1. Strain activation

[0051] The glycerol tube containing Bifidobacterium breve C11 was taken out from the -80°C refrigerator, and the bacterial solution was streaked on the mMRS solid medium, and cultured at 37°C for 48 hours in an anaerobic environment. The grown single colonies were picked and inoculated in mMRS liquid medium, cultured at 37°C for 48 hours under anaerobic environment, and continuously activated for 3 generations.

[0052] 2. Preparation of linoleic acid mother liquor

[0053] Weigh 300mg of linoleic acid (LA) and 200mg of Tween-80, dissolve in water and dilute to 10mL, stir and emulsify thoroughly, filter and sterilize through a 0.45μm sterile filter membrane, and store at -20°C in the dark.

[0054] 3. Co-culture with linoleic acid

[0055] The activated bacterial solution was inoculated into 10 m...

Embodiment 3

[0069] Example 3: Bifidobacterium breve C11 (CCFM683) biotransformation of conjugated linolenic acid

[0070] 1. Strain activation

[0071] The glycerol tube containing Bifidobacterium breve C11 was taken out from the -80°C refrigerator, and the bacterial solution was streaked on the mMRS solid medium, and cultured at 37°C for 48 hours in an anaerobic environment. The grown single colonies were picked and inoculated in mMRS liquid medium, cultured at 37°C for 48 hours under anaerobic environment, and continuously activated for 3 generations.

[0072] 2. Preparation of linolenic acid mother liquor

[0073] Weigh 300mg of α-linolenic acid (α-LNA) and 200mg of Tween-80, dissolve in water and make up to 10mL, stir and emulsify thoroughly, filter and sterilize through a 0.45μm sterile filter membrane, and store at -20°C, protected from light .

[0074] 3. Co-culture with linolenic acid

[0075] The activated bacterial solution was inoculated into 10mL mMRS liquid medium contain...

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Abstract

The invention relates to bifidobacterium breve C11 (CCFM683) and application thereof. According to the invention, bifidobacterium breve C11 (CCFM683) can respectively effectively converter free linoleic acid and linolenic acid into conjugated linoleic acid and conjugated linolenic acid with better biological activity, can be directly used for preparing conjugated linoleic acid or conjugated linolenic acid, and can also be used for producing food rich in conjugated linoleic acid or conjugated linolenic acid.

Description

【Technical field】 [0001] The invention relates to a screened Bifidobacterium breve C11 strain and the application of the strain in preparing conjugated linoleic acid or conjugated linolenic acid. 【Background technique】 [0002] Conjugated linoleic acid (CLA) is the general term for octadecadienoic acid containing conjugated double bonds, and is the positional isomer and geometric isomer of linoleic acid (Linoleic acid, 18:2). The most abundant and common isomer is cis 9, trans 11-CLA (c9, t11-CLA), also known as rumenic acid. In addition, trans 10, cis 12-CLA (t10, c12-CLA) is also an isomer with relatively high content in nature. Conjugated linoleic acid has attracted attention because of its biological functions. Different conjugated linoleic acid isomers have different physiological functions, among which c9,t11-CLA and t10,c12-CLA are recognized as the most physiologically active co- Conjugated linoleic acid isomers, c9, t11-CLA mainly have anti-cancer, anti-inflammato...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/64C12R1/01
CPCC12P7/6427C12N1/205C12R2001/01
Inventor 杨波陈卫陈海琴赵建新张灏陈永泉
Owner JIANGNAN UNIV
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