Method for extracting total anthraquinone from rheum officinale

Abstract

The invention discloses a method for extracting total anthraquinone from rheum officinale. The method comprises the following steps: step one, grinding rheum officinale and sieving; step two, weighing 0.15 part by weight of rheum officinale powder, evenly mixing rheum officinale powder with a proper amount of diatomite, and saving the mixture for later use; step three, adding a little amount of quartz sand into an extraction tank provided with a filter membrane, evenly mixing, adding a proper amount of diatomite into the extraction tank until diatomite reaches the opening of the extraction tank; covering the extraction tank by a cover, carrying out static extraction; and obtaining the liquid extract after extraction; wherein the extraction conditions are as follows: the extracting solvent is acetic acid-methanol (1%), the extraction temperature is 100 DEG C, the static extraction lasts for 5 minutes, the flush volume is 100%, and the static circulation is performed for three times. The method has the advantages that the operation is simple, the extraction time is short, and the requirements on the skills of operators are lowered effectively.

Description

technical field [0001] The invention relates to the field of laboratory testing, in particular to a method for extracting total anthraquinones in rhubarb. Background technique [0002] In 2010, the Pharmacopoeia recorded the method of extracting total anthraquinone in rhubarb: take about 0.15g of the powder of this product (passed through a No. 4 sieve), accurately weigh it, put it in a stoppered Erlenmeyer flask, add 25ml of methanol precisely, weigh it, and heat Reflux for 1 hour, let cool, weigh again, make up the lost weight with methanol, shake well, and filter. Precisely measure 5ml of the filtrate, put it in a flask, evaporate the solvent, add 10ml of 8% hydrochloric acid solution, ultrasonicate for 2 minutes, add 10ml of chloroform, heat and reflux for 1 hour, let it cool, put it in a separatory funnel, and use a small amount of Wash the container with chloroform, put it into a separatory funnel, separate the chloroform layer, extract the acid solution with chlorofo...

Images