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Method for the in vitro micropropagation of plant material and method for large-scale and large-volume production of cloned plant seedlings ready for field growth

A plant material, micropropagation technology, applied in the fields of botanical equipment and methods, plant regeneration, biochemical equipment and methods, etc.

Inactive Publication Date: 2016-09-14
非布利亚塞鲁罗斯股份公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this is still an expensive practice, leaving room for new technologies using equipment and equipment called bioreactors that help produce plants and reduce high-cost labor in large-scale operations

Method used

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  • Method for the in vitro micropropagation of plant material and method for large-scale and large-volume production of cloned plant seedlings ready for field growth
  • Method for the in vitro micropropagation of plant material and method for large-scale and large-volume production of cloned plant seedlings ready for field growth
  • Method for the in vitro micropropagation of plant material and method for large-scale and large-volume production of cloned plant seedlings ready for field growth

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Experimental program
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Embodiment 1

[0079] Embodiment 1—obtain Eucalyptus clone seedling

[0080] The present invention is used to obtain Eucalyptus clone seedling method in bioreactor by intermittent immersion technique and comprises the following steps:

[0081] Step 1 - During the interval between submersion of the plants in the medium and gas exchange of the atmosphere in the internal compartment of the bioreactor, the plant material is kept still. The medium was stored in the explants in the outer container (lower) and inner compartment (upper). The photosynthetic radiation efficient bioreactor consists of a tubular fixture with blue, red and white colored LED lights.

[0082] Step 2 - Activate the solenoid valve by a timer with a preset time and duration within a certain range. This allows compressed air to enter the outer container, increasing the pressure inside the outer compartment (lower), allowing the liquid medium to transfer through the connecting channel between the containers into the inner cha...

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Abstract

The present invention relates to a method for the in vitro micropropagation of plant material, comprising steps of multiplication and elongation of shoots obtained from plant propagules. The invention further includes a method for the large-scale production of cloned plant seedlings ready for field growth, to replace the conventional in vitro clone gardens in biofactories.

Description

technical field [0001] The present invention relates to a method for the large-scale and mass production of clonal plants of arboreal species, in particular of the genus Eucalyptus, whereby a step of vegetative propagation takes place in vitro under in vitro conditions to maximize the yield of arboreal species. Healthy and viable microestacas of this species, while minimizing the rate of vitrification during the micropropagation phase in intermittent submersion protocols. Background technique [0002] Micropropagation, consisting of plant tissue culture techniques used as the basis for seedling formation, is a practical, safe and rapid method of multiplying plants in a reduced space. Micropropagation is conventionally carried out by semi-solid systems, typically in gelling media with agar. However, this remains an expensive practice, leaving room for new technologies using equipment and devices called bioreactors that help produce plants and reduce costly labor in large-sca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12M3/00A01G7/06
CPCA01H4/00A01H4/005A01G22/60A01G31/00
Inventor R·M·彭切尔J·P·D·雷斯M·L·D·奥利韦拉
Owner 非布利亚塞鲁罗斯股份公司