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Extraction method for bioactive polypeptides of bovine placenta

A bioactive polypeptide and extraction method technology, applied in the field of biological extraction, can solve the problems of harsh extraction conditions and complex extraction methods, and achieve the effects of strict process, simple operation and high stability

Inactive Publication Date: 2016-09-21
白城天福粮油食品集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the existing disclosed technical solutions, the extraction method is relatively complicated and the extraction conditions are harsh, therefore, it is necessary to provide a bovine placenta with simple extraction method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The method for extracting bovine placental bioactive polypeptides, the steps are as follows:

[0035] 1) Wash the fresh bovine placenta with water, remove the fetal membranes, large blood vessels, and connective tissue, cut into pieces, add water, and grind it into a 110-mesh homogenate in a tissue homogenizer, in which the addition ratio of placenta to water is 1g: 0.5mL water;

[0036] 2) The obtained homogenate was frozen at -15°C for 8 hours, then thawed at 10°C, and thawed repeatedly in this way for 5 times, and the thawed liquid was collected;

[0037] 3) Add NaOH solution and trypsin to the thawing solution, adjust the pH value to 9, and incubate for 9 hours to obtain the incubating tissue fluid;

[0038] 4) Raise the temperature of the incubating tissue fluid to 55°C and maintain it for 12 minutes;

[0039] 5) Centrifuge the incubated tissue fluid at a centrifugal speed of 2500 rpm for 45 minutes, and take the supernatant, which is the biologically active poly...

Embodiment 2

[0043] The method for extracting bovine placental bioactive polypeptides, the steps are as follows:

[0044] 1) Wash the fresh bovine placenta with water, remove the fetal membranes, large blood vessels, and connective tissue, cut into pieces, add water, and pulverize into 100-120 mesh homogenate, wherein the ratio of placenta to water is 1g: 0.8mL;

[0045] 2) Freeze the homogenate at -18°C for 6 hours, then thaw it at 8°C, repeat the freezing and thawing process 5 times, and collect the thawed liquid;

[0046] 3) Add NaOH solution and trypsin to the thawing solution, adjust the pH value to 9, and incubate for 10 hours to obtain the incubating tissue fluid;

[0047] 4) Raise the temperature of the incubating tissue fluid to 55°C and maintain it for 16 minutes;

[0048] 5) Centrifuge the incubated tissue fluid at a centrifugal speed of 3000 rpm for 50 min, and take the supernatant, which is the biologically active polypeptide extract.

[0049] 6) Suction filter the biologica...

Embodiment 3

[0052] The method for extracting bovine placental bioactive polypeptides, the steps are as follows:

[0053] 1) Wash the fresh bovine placenta with water, remove the fetal membranes, large blood vessels, and connective tissue, cut into pieces, add water, and pulverize into 100-120 mesh homogenate, wherein the ratio of placenta to water is 1g: 0.8mL;

[0054] 2) The homogenate was frozen at -17°C for 6 hours, then thawed at 5-10°C, repeated freezing and thawing 5 times, and the thawed liquid was collected;

[0055] 3) Add NaOH solution and trypsin to the thawing solution, adjust the pH value to 9, and incubate for 8 hours to obtain the incubating tissue fluid;

[0056] 4) Raise the temperature of the incubating tissue fluid to 55°C and maintain it for 15 minutes;

[0057] 5) Centrifuge the incubated tissue fluid at a centrifugal speed of 2800 rpm for 40 minutes, and take the supernatant, which is the biologically active polypeptide extract.

[0058] 6) Suction filter the biol...

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PUM

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Abstract

The invention discloses an extraction method for bioactive polypeptides of a bovine placenta. The extraction method comprises the steps that 1, the fresh bovine placenta is washed clean with water, a placenta membrane, great vessels and connective tissue are removed, and the bovine placenta is cut into blocks, added with water and then crushed into 100-120-mesh homogenate liquid, wherein the ratio of the placenta to the water is 1 g:0.5-1 mL; 2, the homogenate liquid is frozen for 6-8 h at the temperature of minus 15 DEG C to minus 18 DEG C and then melted at the temperature of 5 DEG C to 10 DEG C, freezing and melting are repeatedly conducted for five times, and melt liquid is collected; 3, a NaOH solution and trypsin are added into the melt liquid, the pH is regulated to be 9, and incubating is conducted for 8-10 h to obtain incubating tissue liquid; 4, the temperature of the incubating tissue liquid is increased to 50 DEG C to 60 DEG C and then kept for 10-20 min; 5, centrifuging is conducted for 40-60 min at the centrifuging speed of 2,500 r / min to 3,000 r / min, supernatant liquid is taken, and a bioactive polypeptide extraction liquid is obtained; 6, ultrafiltering and drying are conducted, and bioactive polypeptide powder is obtained. The method is simple in condition, easy to operate and suitable for industrialized production.

Description

technical field [0001] The invention relates to the technical field of biological extraction. More specifically, it relates to a method for extracting biologically active polypeptides from bovine placenta. Background technique [0002] The placenta is rich in all the primordial vital substances necessary for the conception of life. At present, the medicinal and health care effects of placenta, especially bovine placenta, have attracted more and more attention. As far as bovine placenta is concerned, the main components are proteins, hormones and various enzymes, which have the functions of warming the kidney, nourishing essence, nourishing qi, and nourishing blood. To sum up, the functional characteristics of bovine placenta are increasingly recognized by people, and the requirements for further processing of bovine placenta have also been put forward objectively. Since bovine placenta contains a large amount of active substances necessary for the human body, extracting f...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
Inventor 侯继福陈淑芳孙晨
Owner 白城天福粮油食品集团有限公司
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