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Gene GW7 for controlling grain shape, exterior quality and yield of rice and applications of gene GW7

A rice and genetic technology, applied in the fields of botany and biology, can solve the problems of high yield, low quality, neglect of quality improvement, etc.

Pending Publication Date: 2016-10-05
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for a long time, in order to solve the problem of feeding a large population, my country's rice breeding has always taken high yield as the main goal and neglected quality improvement, resulting in the current situation of high yield but not high quality in my country's rice varieties.

Method used

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  • Gene GW7 for controlling grain shape, exterior quality and yield of rice and applications of gene GW7
  • Gene GW7 for controlling grain shape, exterior quality and yield of rice and applications of gene GW7
  • Gene GW7 for controlling grain shape, exterior quality and yield of rice and applications of gene GW7

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0124] Example 1: Acquisition of rice grain type main effect QTL-qGW7

[0125] Minghui 63 and Zhenshan 97 are the parent materials widely used in hybrid rice breeding in my country. Minghui 63 is currently the most widely used restorer line material, and Zhenshan 97A is the most widely used sterile line. Shanyou 63 bred with the two parents is the most popular hybrid rice variety in my country. This variety has excellent yield but poor rice quality. Taifeng A is a high-quality sterile line material bred in South China in recent years. Its grain aspect ratio, chalkiness and other important appearance quality traits have reached the first grade of the national standard.

[0126] The inventor used Zhenshan 97B (bred by Wenzhou Institute of Agricultural Sciences, presented by Mr. Liu Yaoguang, School of Life Sciences, Wushan South China Agricultural University, Tianhe District, Guangzhou) as the female parent and Taifeng A (high-quality rice plant used by the Rice Institute of Gua...

Embodiment 2

[0133] Embodiment 2: Obtaining of rice grain type gene GW7

[0134] The inventors used Taifeng A (Guangdong Provincial Academy of Agricultural Sciences Rice Institute to use the high-quality rice line Mi 31 and (Bo B / Zhe 9248) F8 high-quality line to cross, and then through 8 generations of backcrossing to produce a high-quality indica rice male sterile line ) and Huajingxian 74 (a high-yielding rice variety bred by the Department of Agronomy of South China Agricultural University, approved in 2000 in Guangdong Province, and presented by Mr. Zhang Guiquan from the Agricultural College of Wushan South China Agricultural University, Tianhe District, Guangzhou City) and combined with continuous multi-generation backcross Constructed BC 3 f 2 group. On this basis, the GW7 gene was cloned using the map-based cloning technique. The polymorphic marker primer sequences used in the map-based cloning are shown in Table 3, and the detection method of the polymorphic marker is as descri...

Embodiment 3

[0138] Example 3: Construction of near isogenic line materials NIL-GW7 and NIL-gw7

[0139] The BC described by the inventor in Example 2 3 f 2 The individual plants containing qGW7 and slender grain were selected in the population and continued to be backcrossed with Huajingxian 74 for multiple generations to BC 6 f 2， Individual plants were selected according to the phenotype of elongated and narrowed grains, and molecular markers were used to track and detect the target segment, and background scanning was performed on the segments other than the target traits, and finally the genetic background of Huajingxian 74 (NIL-gw7) was obtained. The very close near-isogenic line NIL-GW7 material was used for follow-up research. The molecular marker detection method is the same as that described in Example 1.

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Abstract

The invention discloses a gene GW7 (SEQ ID NOs:1-4) for controlling the grain length and the grain width of rice and applications of the gene GW7. GW7 can control the grain length, the grain width and the grain length-width ratio of the rice, so that the grain quality of the rice is improved; after polymerization of GW7 and gs3, the yield of the rice can be obviously improved, and meanwhile, the quality of the rice can be improved. The invention also discloses a method for breeding crops with the increased yield by utilizing the gene GW7, and a marker-assisted selection pyramiding breeding method for breeding rice variety with the improved rice quality and yield. The gene GW7 has a wide application prospect in the high-yield and high-quality breeding of the crops such as the rice.

Description

technical field [0001] The present invention belongs to the fields of botany and biotechnology. Specifically, the present invention relates to a gene GW7 for controlling grain shape and appearance quality of rice and its application. The present invention also relates to the polypeptide encoded by the gene and the promoter sequence of the gene. Background technique [0002] Rice (Oryza sativa L.) is the largest food crop in my country and one of the main food crops in the world. Nearly half of the world's population uses rice as a staple food. In recent years, the rice planting area has continued to decline. In order to solve the problem of food shortage, increasing the rice yield per unit area has become the most important way to increase the total grain output. Rice semi-dwarf breeding and utilization of heterosis have greatly increased the yield of rice and made a great contribution to ensuring my country's food security. However, for a long time, in order to solve the...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/63C12N1/21C12N5/10C12N15/84C12N15/113C12Q1/68A01H5/00C12R1/19C12R1/01
Inventor 傅向东王少奎吴昆刘倩李姍
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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