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Kit for detecting methylation degree of ndrg4 gene related to gastric cancer and application thereof

A methylation and kit technology, which is applied in the field of kits for the methylation degree of NDRG4 gene, can solve the problem that there is no public detection kit for detecting the methylation degree of CpG island at the 3' end of NDRG4 gene, etc., and achieve the detection result. Accurate and reliable, short cycle, highly targeted effect

Active Publication Date: 2020-01-07
NINGBO UNIV
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  • Abstract
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Problems solved by technology

At present, there are no relevant research reports on the use of pyrosequencing to detect the degree of methylation of the CpG island at the 3' end of the NDRG4 gene in gastric cancer.

Method used

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  • Kit for detecting methylation degree of ndrg4 gene related to gastric cancer and application thereof
  • Kit for detecting methylation degree of ndrg4 gene related to gastric cancer and application thereof
  • Kit for detecting methylation degree of ndrg4 gene related to gastric cancer and application thereof

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Embodiment Construction

[0019] The present invention will be described in further detail below in conjunction with the embodiments of the drawings.

[0020] 1. Collection of research objects and samples

[0021] Collected 110 cases of gastric cancer tissues and corresponding paired normal tissues adjacent to cancer from December 2007 to February 2010 in a hospital in Hangzhou City, Zhejiang Province. The cancer tissue was taken from the center of the tumor tissue and the distance between the adjacent tissues The cancerous tissue was more than 5 cm and was non-cancerous tissue. All cases had not received radiotherapy and chemotherapy before surgery and were confirmed by histopathological examination. Patients aged 21 to 83 years old, of which 76 were males and 34 were females; 30 had a history of smoking and 80 had no history of smoking; 28 had a history of drinking, 82 had no history of drinking; tumor size: ≥6cm 43 cases, <6cm 67 cases. According to the American Joint Committee on Cancer (AJCC) TNM stag...

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Abstract

The invention discloses a kit for detecting the gastric cancer related NDRG4 gene methylation degree and an application thereof. The kit is characterized by a pair of NDRG4 gene methylation specific amplification primers and a methylation specific sequencing primer, wherein the nucleotide sequence of the forward primer is 5'-AGGGTTGGGGGTTTTAGA-3'; the nucleotide sequence of the reverse primer is 5'-Biotin-CACCCTCTACCAAAAACTCAAAACTCAATT-3'; and the nucleotide sequence of the sequencing primer is 5'-GGGGTTTTAGAGTGTAT-3'. The kit has the advantages of easiness and convenience in detection, strong targeting property and high detection accuracy and efficiency and is used for preparing a reagent or kit for early diagnosis of gastric cancer.

Description

Technical field [0001] The invention relates to a method for assisting the early diagnosis of gastric cancer, in particular to a kit for detecting the methylation degree of NDRG4 gene related to gastric cancer and its application. Background technique [0002] Gastric cancer is a malignant tumor originating from the epithelium of the stomach and is one of the most common malignant tumors worldwide. Its morbidity and fatality rate ranks 4th and 3rd in the world, and it is a disease that seriously threatens human life and health. In China, the incidence of gastric cancer is 29.9 / 100,000, and the mortality rate is 22.3 / 100,000. Compared with other countries in the world, men and women in Chinese gastric cancer population rank first in the world with the highest cancer mortality. With the development of medical methods and the popularization of health concepts, although the incidence and mortality of gastric cancer are on the decline, the overall prognosis is still poor. Due to th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/118C12Q2600/154
Inventor 段世伟陈晓颖
Owner NINGBO UNIV