Extraction method of diatom polysaccharides
A diatom polysaccharide and extraction method technology, applied in the field of polysaccharide extraction, can solve the problems of low extraction rate, restrict the industrial application of diatom polysaccharide, complex extraction method, etc., and meet the requirements of simple process, good practicability, industrialization and equipment. low effect
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Embodiment 1
[0020] Using Chaetoceros (a kind of diatom) dry powder as raw material, proceed as follows:
[0021] 1. Add 100g Chaetoceros dry powder (moisture content about 14%) into 2kg deionized water, place in 80°C water bath and extract while stirring for 30 minutes, and use a centrifuge to separate the supernatant liquid and slag at a speed of 3000 R / min or more , and the filter residue was extracted 2 times in the same way again. The extracts obtained from the three extractions were combined.
[0022] 2. Concentrate the resulting extract by rotary vacuum distillation at 80° C. until the volume is reduced to one tenth of its original volume.
[0023] 3. Use a centrifuge to centrifuge the concentrated solution at a speed of 3000 R / min to remove the solid residue and keep the upper liquid.
[0024] 4. Take the supernatant, add three times the volume of absolute ethanol, and precipitate naturally. The precipitate is diatom polysaccharide, which can be centrifuged to obtain diatom polys...
Embodiment 2
[0027] The method takes the chaetoceros hygrocystis in diatoms as raw material, and carries out according to the following steps:
[0028] 1. Add 1000g of Chaetoceros (a type of diatom, with a water content of about 90%) wet algae to 1kg of deionized water, place it in a water bath at 85°C and extract it for 40 minutes while stirring. The supernatant liquid and residue were separated, and the filter residue was extracted twice in the same way. The extracts obtained from the three extractions were combined.
[0029] 2. Concentrate the resulting extract by rotary vacuum distillation at 85° C. until the volume is reduced to one tenth of its original volume.
[0030] 3. Use a centrifuge to centrifuge the concentrated solution at a speed of 4000 R / min to remove the solid residue and keep the upper liquid.
[0031] 4. Take the supernatant, add 4 times the volume of absolute ethanol, and the precipitate will naturally precipitate out. The precipitate is diatom polysaccharide, which...
Embodiment 3
[0034] Using Chaetoceros (a kind of diatom) dry powder as raw material, proceed as follows:
[0035] 1. Add 100g Chaetoceros dry powder (moisture content about 14%) into 1.5kg deionized water, place in a 60°C water bath and extract while stirring for 25 minutes, and use a centrifuge to separate the supernatant and scum.
[0036] 2. Concentrate the resulting extract by rotary vacuum distillation at 83°C until the volume is reduced to one-eighth of its original volume.
[0037] 3. Use a centrifuge to centrifuge the concentrated solution at a speed of 2000 R / min to remove the solid residue and keep the upper liquid.
[0038] 4. Take the supernatant, add twice the volume of absolute ethanol, and precipitate naturally. The precipitate is diatom polysaccharide, which is centrifuged at low temperature and vacuum-dried to obtain 9.1 g of diatom polysaccharide.
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