Carbon quantum dot/aurum cluster ratiometric fluorescent probe for detection of cadmium ion and ascorbic acid

A ratiometric fluorescent probe, ascorbic acid technology, applied in the field of fluorescent sensing, can solve the problems of high cost and weak luminescence stability, and achieve high selectivity, sensitivity, and high detection accuracy
CN106047342AInactive Publication Date: 2016-10-26NANJING UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANJING UNIV OF SCI & TECH
Publication Date
2016-10-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a carbon quantum dot / aurum cluster ratiometric fluorescent probe for detection of cadmium ion and ascorbic acid. A preparation method comprises the following steps: preparing CQDs (Carbon Quantum Dots) from alanine and histidine through a one-step hydrothermal method; performing amino-functionalization on the CQDs by using 3-aminopropyltriethoxysilane, wherein the CQDs which are subjected to the amino-functionalization serve as a reference chromophore; reducing chloroauric acid through sodium borohydride by taking 11-sulfydryl undecanoic acid as a surfactant to obtain MUA-modified AuNCs (Aurum Nano Clusters), wherein the MUA-modified AuNCs serve as a main fluorophore of the ratiometric fluorescent probe; finally, coupling the CQDs and the AuNCs through an amidation reaction to obtain the CQDs / AuNCs ratiometric fluorescent probe. On the basis of static quenching and inter-filtering effects, the fluorescence of the CQDs / AuNCs can be quenched by Cd<2+>; the invention discloses the application of the CQDs / AuNCs ratiometric fluorescent probe in Cd<2+> detection. The fluorescence of the CQDs / AuNCs which is quenched by the Cd<2+> can be recovered by the ascorbic acid, so that the ratiometric fluorescent probe can also be used for the detection of AA (Ascorbic Acid). The ratiometric fluorescent probe disclosed by the invention has the lower detection limit of 32.5 nM to the Cd<2+>, has the lower detection limit of 0.105 muM to the AA, and has an application value in the detection of the cadmium ion and the ascorbic acid.
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Description

technical field

[0001] The invention belongs to the technical field of fluorescent sensing, in particular to a carbon quantum dot / gold cluster ratio fluorescent probe for detecting cadmium ions and ascorbic acid and a preparation method thereof. Background technique

[0002] Ratio-type fluorescent probes have a main fluorophore and a reference chromophore, and the two emission peaks are separated. The ratio of fluorescence intensity can provide internal correction of environmental interference and eliminate fluctuations in excitation light intensity, improving the accuracy of quantitative analysis. Breakthrough The probe based on a single fluorescence intensity is easily affected by the detection substrate, photobleaching and other shortcomings, and at the same time avoids the interference of probe concentration, polarity, environmental pH and other factors, and solves the dilemma of false positive consequences. It has received great attention in recent years.

[0003] Cadm...

Claims

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