Molecular marker for aiding selection of Rf (fertility restorer) gene, specific primers and application

A technology of molecular markers and gene selection, which is applied in biochemical equipment and methods, microbiological measurement/inspection, DNA/RNA fragments, etc., can solve the problems of long breeding cycle, rare, and limited pepper CMS application, etc., to achieve The effect of saving manpower and material resources and accurate judgment

Active Publication Date: 2016-10-26
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0003] The restoration of male sterility in pepper is mainly controlled by the main gene Rf (Fertility restorer) located on chromosome 6 of pepper. Th

Method used

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  • Molecular marker for aiding selection of Rf (fertility restorer) gene, specific primers and application
  • Molecular marker for aiding selection of Rf (fertility restorer) gene, specific primers and application
  • Molecular marker for aiding selection of Rf (fertility restorer) gene, specific primers and application

Examples

Experimental program
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Embodiment 1

[0016] A BC constructed by backcrossing pepper sterile parent 77013A, fertile parent 0601M and its F1 with 77013 multiple times 5 f 2 The population ((77013A×0601M)×77013) was the test material.

[0017] Genetic Population Construction of Capsicum Rf Gene

[0018] F1 was obtained by crossing the pepper sterile parent 77013A (Capsicum annuum L.) and the pepper restorer line material 0601M (Capsicum annuum L.) as parents, and the BC obtained by backcrossing F1 and 77013 multiple times 5 f 2 The population ((77013A×0601M)×77013) is a mapping population, with a total of 741 individual plants.

[0019] 1. Genomic DNA extraction

[0020] Genomic DNA of all individual BC5F2 plants, parents and F1 was extracted by CTAB method, the concentration and quality were measured by Biospec-nano micro-spectrophotometer, and the concentration was diluted to 100ng / μl.

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Abstract

This invention relates to the field of molecular markers, in particular to a molecular marker for aiding selection of a Rf (fertility restorer) gene, specific primers and an application. The genetic distance between the molecular marker and the Rf gene is 0.7cM, and the molecular marker is closely linked with the gene. Specific upstream and downstream primers of the molecular marker are as follows: the sequence of the upstream primer of MI16 is CCTCGAATATAAACTTATGGTGTCC, and the sequence of the downstream primer of MI16 is GCTTGGCAACCTATTCTTAGAAGTT. The M16 marker can quickly identify the Rf gene in a sweet pepper, and better complement the study of the sweet pepper in the direction of male sterility.

Description

technical field [0001] The invention relates to the field of molecular markers, in particular to molecular markers, specific primers and applications for assisting Rf gene selection. Background technique [0002] The use of cytoplasmic male sterility (CMS, Cytoplasmic male sterility) three-line hybrid seed production is an effective method to save the cost of artificial detasseling in pepper seed production. Since the production of pepper is based on the fruit, if the male sterile restorer line of the male parent is not strong in fertility recovery, it will lead to insufficient fruit seeds, which will seriously affect the hybrid F. 1 The appearance and yield of commercial pepper fruit. Therefore, the breeding of cytoplasmic male sterile male parent (restorer line) with strong restorative ability is one of the key issues in the utilization of pepper CMS. [0003] The restoration of male sterility in pepper is mainly controlled by the main gene Rf (Fertility restorer) locate...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 张正海王立浩张宝玺曹亚从朱砚姝
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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