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Distinguishing method for bacillus subtilis and bacillus amyloliquefaciens

A technology of Bacillus subtilis and Bacillus amyloliquefaciens, which is applied in the field of distinguishing similar Bacillus, can solve problems such as difficulty in distinguishing Bacillus subtilis and Bacillus amyloliquefaciens, and achieves the effects of excellent identification, accurate discrimination and simple method.

Inactive Publication Date: 2016-10-26
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention provides a method for distinguishing Bacillus subtilis and Bacillus amyloliquefaciens in order to solve the problem that it is difficult to distinguish Bacillus subtilis and Bacillus amyloliquefaciens in the existing strain classification method

Method used

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  • Distinguishing method for bacillus subtilis and bacillus amyloliquefaciens
  • Distinguishing method for bacillus subtilis and bacillus amyloliquefaciens
  • Distinguishing method for bacillus subtilis and bacillus amyloliquefaciens

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Experimental program
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specific Embodiment approach 1

[0017] Specific embodiment one: the method for distinguishing Bacillus subtilis and Bacillus amyloliquefaciens in this embodiment is carried out according to the following steps:

[0018] 1. Obtain the CheA gene of the Bacillus to be identified;

[0019] 2. Comparing the homology with the CheA gene of the known type strains of Bacillus subtilis and Bacillus amyloliquefaciens, the homology lower than 80% is heterogeneous Bacillus.

specific Embodiment approach 2

[0020] Specific embodiment two: the difference between this embodiment and specific embodiment one is: Bacillus subtilis subsp.subtilis str.168 is selected as the model strain of known Bacillus subtilis in step two; Bacillus amyloliquefaciens subsp.plantarum str.FZB42 is selected as the already The type strain of Bacillus amyloliquefaciens. Other steps and parameters are the same as those in Embodiment 1.

[0021] The whole genome accession number of Bacillus subtilis subsp.subtilis str.168 is GI:728882887; the whole genome accession number of Bacillus amyloliquefaciens subsp.plantarum str.FZB42 is GI:154350369.

Embodiment 1

[0023] This example uses BLASTN 2.2.30+ to compare the homology among the 16SrRNA, GyrA, GyrB, CheA and GroEL genes of the genomes of the model strains (Bacillus subtilis subsp. subtilis str.168 and Bacillus amyloliquefaciens subsp. plantarum str. FZB42).

[0024] This implementation model strain comparison dot matrix analysis (Dot Matrix View) diagram as shown figure 1 shown. The results of the homology test between the genes of the model strains are shown in Table 1. The results of comparative analysis of the type strains were as follows: figure 2 shown.

[0025] Table 1

[0026] Gene

16SrRNA

GyrA

GrB

CheA

GroEL

homology

99％

81％

82％

77％

93％

[0027] figure 1 and figure 2 It shows that there is a difference between Bacillus subtilis subsp.subtilis str.168 and Bacillus amyloliquefaciens subsp.plantarum str.FZB42, 80% of the nucleic acids are homologous, and the other 20% of the sequences are significantly differen...

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Abstract

A distinguishing method for bacillus subtilis and bacillus amyloliquefaciens relates to distinguishing methods for similar bacillus. The method includes the steps of: 1) acquiring a CheA gene of the to-be-distinguished bacillus; 2) performing homologous comparison with the CheA genes of known mode strains of the bacillus subtilis and bacillus amyloliquefaciens, and if the homology is lower than 80%, determining the to-be-distinguished bacillus to be heterogenous bacillus. The method distinguishes the bacillus subtilis and the bacillus amyloliquefaciens through the CheA gene and is simple and accurate.

Description

technical field [0001] The invention relates to a method for distinguishing related bacillus. Background technique [0002] The Polyphasic taxonomy method proposed by Colwell in 1970 is the most effective means to distinguish taxa at all levels in the field of microorganisms, and it is also a classification method that more objectively reflects the evolutionary relationship of natural systems among organisms. There are three types of data and information in this classification method: phenotype, genotype, and phylogenetic type. Phenotype information includes: analysis of culture morphology, physiological and biochemical indicators, chemical composition of cell wall, and cytochemistry of phospholipids, quinones, and whole-cell fatty acids, as well as electrophoresis of whole-cell soluble proteins and ribosomal protein maps; genotype information It comes from the analysis of cellular nucleic acid molecules, GC content determination, nucleic acid hybridization, restriction fra...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04C12R1/125C12R1/07
CPCC12Q1/689
Inventor 赵晓宇王佳龙于冲沙长青孟利强
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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