Method for directly sowing seeds of dendrobium devonianum and cultivating seedlings on tea trees

A technology of Dendrobium denticola and seeds, which is applied in the field of Dendrobium cultivation, can solve problems such as the immaturity of wild ecological seedling raising technology, and achieve the effects of simplifying the production process of seedlings, increasing the amount of seedlings, and improving environmental adaptability.

Active Publication Date: 2016-11-09
JINGHONG HONGZHEN AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the field ecological seedling raising technology about Dendrobium dendrobii in the prior art is still immature

Method used

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  • Method for directly sowing seeds of dendrobium devonianum and cultivating seedlings on tea trees
  • Method for directly sowing seeds of dendrobium devonianum and cultivating seedlings on tea trees

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Acquisition and preservation of dental flap germplasm

[0029] The seeds of Dendrobium dendrobium that are naturally strong and mature but not yet cracked are collected and stored for later use. The Dendrobium dendrobium seeds used were collected from a wild population in Longling County, Baoshan City, Yunnan Province.

[0030] 2. Production of fungus suspension

[0031] 2.1 The production of culture medium: the conventional method of PDA liquid culture medium, appropriate amount. Divide each bottle of 50ml into 70 500ml triangular flasks. After sealing, wrap the mouth of the bottle with newspaper, put it into a sterilization pot and sterilize at 121°C and 115KPa for 20 minutes, and then set aside.

[0032] 2.2 The strain-Tulasnella sp. CGMCC No. 9551, which was activated and cultured for 7 days, was inoculated into the sterilized Erlenmeyer flask containing 50ml liquid medium, and fermented and cultured at 28°C and 150rpm for 7 days.

[0033] 2.3 Use gauze to filter the cul...

Embodiment 2

[0045] Repeat Example 1, with the following differences:

[0046] Planting was carried out at Longpa Ancient Tea Garden in Yanuo Village, Jinuo Township, Jinghong City, Yunnan Province as a test site on June 17, 2014. The seed density of the seed-fungus suspension is 118 / ml, totaling 136ml.

[0047] Most protocorms develop into seedlings at 45-50 days after sowing. A total of 34 sowing spots were selected for this sowing, of which 18 spots had seeds germinated to form seedlings, with a population germination rate of 52.94%; a total of 128 seedlings were obtained, with an average of 3.76 seedlings per spot.

Embodiment 3

[0049] Repeat Example 1, with the following differences:

[0050] On July 5, 2014, planting was carried out at Yibi Ancient Tea Garden in Yiwu Township, Mengla County, Yunnan Province as a test site. The seed density of the seed-fungus suspension is 88 / ml, a total of 180ml.

[0051] Most protocorms develop into seedlings at 45-50 days after sowing. A total of 45 sowing spots were selected for this sowing, of which 10 spots had seeds germinated to form seedlings, the population germination rate was 22.22%; a total of 49 seedlings were obtained, with an average of 1.09 seedlings per spot.

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Abstract

The invention discloses a method for directly sowing seeds of dendrobium devonianum and cultivating seedlings on tea trees. The method includes preparing fungus suspension from Tulasnella sp. and uniformly mixing the fungus suspension with the seeds of the dendrobium devonianum to obtain seed-fungus suspension; winding preservative films on each trunk of each tea tree by 1-3 circles, uniformly dispersing and injecting the seed-fungus suspension in gaps between the preservative films and the trunks and forming holes in positions near seed sowing points by means of pricking. The seeds of the dendrobium devonianum are collected from fields. The method has the advantages that seedling production procedures can be simplified, the production cost can be obviously lowered, the seedling quantities can be increased, the survival rate and the growth speeds of the seedlings can be increased after the seedlings return natural environments, and accordingly the seedlings are excellent in environmental adaptability; dendrobium devonianum products can be harvested by local residents while tea leaves are collected, and accordingly income increasing effects can be realized; extra land occupation, cultivation facility construction and chemical fertilizer and pesticide usage can be omitted, only the extremely low cost input for preparing the seed-fungus suspension is required, and accordingly the method has extremely high popularization and application value.

Description

Technical field [0001] The invention belongs to the technical field of dendrobium cultivation, and specifically relates to a method for directly sowning Dendrobium dendrobium seeds on a tea tree trunk based on a symbiotic germination technique to obtain seedlings of Dendrobium dendrobium. Background technique [0002] Dendrobium devonianum (Dendrobium devonianum) stems contain high polysaccharides. As a traditional Chinese medicinal material, it has extremely high medicinal value. It is widely cultivated in many areas of Yunnan, especially in Longling County, which has become its pillar industry. The existing cultivation mode is mainly intensive planting, mostly adopting the form of "company + base + farmer", using tissue culture seedlings as seedlings, and the need to build greenhouses, sprinkler irrigation systems, and land occupation rents make the planting costs high At the same time, in the growth process of dendrobium seedlings, in order to promote the rapid growth of dendr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00
CPCA01G31/00
Inventor 高江云邵士成黄晖刘强
Owner JINGHONG HONGZHEN AGRI TECH CO LTD
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