Cell culture bottle with support

Abstract

The invention relates to a cell culture bottle with a support. The cell culture bottle with the support comprises a bottle body, a bottle neck and the support, wherein the bottle neck is positioned on the bottle body; the bottle body is formed by four side walls which are opposite in pairs in a surrounding manner; the side walls comprise a front side wall and a back side wall which are opposite; one side, which faces the front side wall, of the bottle neck is inclined; a bottle opening at the upper end of the bottle neck is inclined upwards; the support comprises a vertically distributed support plate, a plurality of sliding seats and hooks; the sliding seats are distributed on the top surface of the support plate in the length direction, and are glidingly connected to the support plate; positioning bolts are in threaded connection to the sliding seats; the hooks are fixedly connected to one sides of the sliding seats; each hook comprises a clamping part inclined to the direction of the corresponding sliding seat at an angle of 45 degrees and a connecting part, and the bottom ends of the clamping parts are fixedly connected to the sliding seats through the connecting parts; a U-shaped sleeve with a downward opening is fixed on the side wall of the back surface of the bottle body; the bottle body is matched with the clamping parts in a clamped manner through the U-shaped sleeve and then is propped against the support. The cell culture bottle with the support is convenient to operate and diversified in function.

Description

technical field [0001] The invention relates to the technical field of cell culture bottles, in particular to a cell culture bottle with a support. Background technique [0002] In the process of cell culture, rectangular parallelepiped flasks are widely used at present. The digestion and passage of cells are operated in ultra-clean benches or biological safety cabinets according to requirements. These two operating platforms are local purification for experimenters to operate. The equipment, through the circulating air flow, achieves local ultra-low sedimentation bacteria, creates a local high-clean air environment, and greatly reduces the probability of bacterial contamination during cell culture. It is a necessary special equipment for scientific research, pharmaceuticals, and medical and health operations. [0003] According to the characteristics of different types of cells, cell culture can be roughly divided into adherent cell culture and suspension cell culture. Dur...

AI Technical Summary

Problems solved by technology

This step needs to be performed in an ultra-clean bench. The experimenter needs a hand-held culture bottle. After opening the bottle cap, use a pipette or a pipette gun to add trypsin. During this period, the pipette needs to be changed or the trypsin container needs to be opened. , often requires two-handed operation, and the culture bottle is often placed on the table vertically with the open mouth, but the airflow in the ultra-clean bench is vertical from top to bottom, while the oblique opening of the culture bottle is upward, so there is a certain probability that it will be brought by the airflow Bacterial contamination caused by the outside or the operator's hand

In the next step of cell subculture after the adherent cells are suspended or the suspension cells are subcultured, the cells in the original culture flask need to be inoculated into several new culture flasks respectively. During the process of transferring cells out of the cell culture bottle, the other culture bottle can only be placed vertically on the table, and the movement of the operator's hand in the ultra-clean table disturbs the vertical air flow, causing bacteria to easily enter the bottle mouth and increase the Chance of infection

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