Uc002mev.3 and in-vitro detection reagent, preparation or kit, application and detection method

An in vitro detection and kit technology, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the problems of false elevation and achieve the effect of enriching research

Inactive Publication Date: 2016-11-09
THE FIRST TEACHING HOSPITAL OF XINJIANG MEDICAL UNIVERCITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, troponin I (cTnI) is a widely used clinical biomarker for the diagnosis of acute myocardial infarction, but the plasma cTnI concentration may be in some cardiac and non-cardiac diseases, such as severe heart failure, atrial fibrillation, chronic kidney disease , severe sepsis and septic shock

Method used

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  • Uc002mev.3 and in-vitro detection reagent, preparation or kit, application and detection method
  • Uc002mev.3 and in-vitro detection reagent, preparation or kit, application and detection method
  • Uc002mev.3 and in-vitro detection reagent, preparation or kit, application and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: A long-chain non-coding RNA uc002mev.3, the nucleic acid sequence of which is shown in SEQ ID No:1. The transcription region of the long-chain non-coding RNA uc002mev.3 (lncRNA uc002mev.3) described in this example is located on chromosome 19, with a starting position of 6,436,431-6,459,781 and a full length of 23,351 bp. The long-chain non-coding RNA uc002mev.3 described in this example can provide new blood biomarkers and therapeutic targets for the early diagnosis and prognosis monitoring of acute myocardial infarction, further enriching the research on the pathogenesis of acute myocardial infarction.

Embodiment 2

[0023] Example 2: As an optimization of the above example, the acute myocardial infarction-related gene is the full length of the long-chain non-coding RNA uc002mev.3.

Embodiment 3

[0024] Example 3: A reagent for detecting long-chain non-coding RNA uc002mev.3 in vitro, including a pair of specific primers for amplifying long-chain non-coding RNA uc002mev.3, the pair of specific primers includes upstream primers and downstream primers, upstream The sequence of the primer is shown in SEQ ID No: 2, and the sequence of the downstream primer is shown in SEQ ID No: 3. The expression level of lncRNA uc002mev.3 described in the present invention can be obtained by combining the reagents for in vitro detection of long-chain non-coding RNA uc002mev. The expression level of lncRNA uc002mev.3 can be used for screening of acute myocardial infarction. The specific primer pair described in this example is applicable to the detection of SYBRGreen, Taqman probes, molecular beacons, double-hybrid probes, and composite probes.

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Abstract

The invention relates to the technical field of biological engineering myocardial infarction related gene, and relates to a lncRNA uc002mev.3 and in-vitro detection reagent, preparation or kit, an application and a detection method; the long non-coding RNA uc002mev.3 has a nucleic acid sequence as shown in SEQ ID No:1. The long non-coding RNA uc002mev.3 can provide new blood biomarkers and therapeutic targets for early diagnosis and prognosis monitoring of acute myocardial infarction, and further enriches researches on a pathogenesis of acute myocardial infarction.

Description

technical field [0001] The invention relates to the technical field of bioengineering myocardial infarction-related genes, and is a reagent, preparation or kit for in vitro detection of uc002mev.3 and its application and detection method, namely lncRNA uc002mev.3 and its reagent, preparation or kit for in vitro detection , application, detection method. Background technique [0002] Acute myocardial infarction (AMI) is a cardiovascular disease that seriously threatens human health. Its morbidity and mortality have been on a significant upward trend in recent years, seriously threatening people's life and quality of life. There are at least 500,000 new cases each year in my country, and at least 2 million existing cases. Acute myocardial infarction (AMI) is the acute necrosis of myocardial tissue caused by persistent severe ischemia. Early diagnosis can improve the survival rate of patients with myocardial infarction and can improve the long-term prognosis. At present, trop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12Q1/68
CPCC12N15/113C12N2310/10C12Q1/6883C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 杨毅宁翟慧马依彤李晓梅刘芬陈邦党谢翔王雪梅陈清杰
Owner THE FIRST TEACHING HOSPITAL OF XINJIANG MEDICAL UNIVERCITY
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