Kit for rapidly coupling proteins, antibodies, enzyme and nucleic acid and preparation method of kit

Abstract

The invention relates to a kit for rapidly coupling proteins, antibodies, enzyme and nucleic acid. The kit comprises test tubes which are respectively filled with magnetic microspheres, washing liquid, a coupling buffer solution, a closing buffer solution and a preserving buffer solution, wherein the magnetic microspheres are prepared by the following steps: (1) respectively sieving 500mL of N,N-dimethylacetamide (DMAC) and 500mL of isopropanol by using a 4A molecular sieve; (2) washing carboxyl magnetic beads for 2-3 times with DMAC; (3) adding DMAC and N-hydroxysuccinimide into the washed carboxyl magnetic beads, and reacting at 40 DEG C for 0.5h; and (4) collecting reaction products, washing twice with isopropanol, finally adding isopropanol to prepare 10mg / mL activated magnetic bead suspension liquid, and preserving at 4 DEG C. According to the kit, the problems of tedious and complex operations of a user in processes of magnetic bead activation, ligand coupling, magnetic bead closing and the like are solved, the operation steps are simplified, and reasonable utilization of resources is realized.

Description

technical field

[0001] The invention belongs to the field of biological kit materials, and in particular relates to a kit for coupling proteins, antibodies, enzymes and nucleic acids and a preparation method thereof, which can rapidly, simply and efficiently combine proteins, antibodies, enzymes and amino-modified nucleic acids And other molecules with amino groups are covalently coupled to activated magnetic microspheres, and the magnetic beads after coupling ligands can be used for detection, separation and purification, etc. Background technique

[0002] Immunomagnetic beads are a new immunological technology developed in recent years. It combines the unique advantages of solidified reagents with the high specificity of immunological reactions. Based on immunology, it penetrates into pathological, physiological, pharmacological, microbial It has been widely used in immunoassay, cell separation, purification of biological macromolecules and molecular biology.

[0003] Usi...