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Breast cancer diagnosis kit based on combined detection of FGF1, FGF10 and IL6

A diagnostic kit and breast cancer technology, applied in the field of protein detection, to achieve the effects of low cost, good accuracy, and widening the optional space

Inactive Publication Date: 2016-11-09
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention improves the specificity and accuracy of breast cancer diagnosis based on the combined detection of FGF1, FGF10 and interleukin IL6, and no such report has been seen in the field

Method used

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  • Breast cancer diagnosis kit based on combined detection of FGF1, FGF10 and IL6
  • Breast cancer diagnosis kit based on combined detection of FGF1, FGF10 and IL6
  • Breast cancer diagnosis kit based on combined detection of FGF1, FGF10 and IL6

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Example 1. Selection and verification of the markers described in the present invention

[0039] The detection of FGF10 used the ELISA kit of Uscn Company. from figure 1 We can clearly see the differences between the three groups of samples. The serum FGF10 concentration of healthy people is 104-135pg / ml, the concentration of benign tumors is between 158-208pg / ml, and the serum concentration of malignant tumors is 158-208pg / ml. The concentration is 318-575pg / ml. Statistical analysis showed that benign vs malignant P=0.004<0.05, malignant vs healthy P=0.0121<0.05 were significantly different.

Embodiment 2

[0040] Embodiment 2, kit of the present invention and preparation thereof

[0041] This embodiment provides a diagnostic kit for breast cancer, which includes a reagent for quantitative detection of fibroblast growth factor-10.

[0042] Specifically, the agent is an antibody to fibroblast growth factor 10.

[0043] Further, the diagnostic kit for breast cancer also includes conventional reagents used in enzyme-linked immunosorbent assay.

[0044] This embodiment also provides a preparation method of a breast diagnostic kit, comprising the following steps: assembling an antibody for fibroblast growth factor 10 into the kit; and / or

[0045] Antibody to fibroblast growth factor 10 is placed in the package marked breast cancer diagnostic use.

[0046] The steps further include: the reagents for assembling the kit also include conventional reagents used in ELISA; and / or

[0047] The conventional reagents used in the enzyme-linked immunosorbent assay are also placed in the packag...

Embodiment 3

[0050] Embodiment 3, adopt kit described in the present invention to carry out breast cancer diagnosis

[0051] Such as Figure 1-Figure 3 As shown, by detecting the concentrations of IL6, IL9, IL21, FGF1, FGF10, and FGF14 in the serum samples of healthy, benign, and malignant groups, we finally selected a group that can be detected in most of the samples and is detected in malignant patients and healthy controls. IL6, FGF1, and FGF10, which had significant differences between groups, were used as markers for the diagnosis of breast cancer. In addition, we also detected the now-recognized tumor marker CEA in serum as a positive control. The data showed that in samples No. 1, 545400, No. 50, and No. 48 of which CEA was determined to be breast cancer, FGF1 , FGF10 or IL6 concentration is also quite high. And in samples No. 39, No. 45, No. 494969, and No. 349922 whose CEA concentration was within the normal range, one or both of IL6, FGF1, and FGF10 were also higher than normal...

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Abstract

The invention provides a breast cancer diagnosis kit based on combined detection of fibroblast growth factors FGF1 and FGF10 and interleukin IL6, and belongs to the field of protein detection. The breast cancer diagnosis kit comprises reagents for quantitatively detecting the FGF1, the FGF10 and the interleukin IL6. The invention also provides a preparation method of the kit. The kit for clinically diagnosing breast cancer has the advantages of convenience in operation, low cost, good specificity and good accuracy.

Description

technical field [0001] The invention belongs to the field of protein detection, in particular to a breast cancer diagnostic kit based on joint detection of FGF1, FGF10 and IL6. Background technique [0002] The female mammary gland is composed of skin, fibrous tissue, mammary glands and fat. Breast cancer is a malignant tumor that occurs in the mammary gland epithelial tissue. The global incidence of breast cancer has been on the rise since the late 1970s. 1 in 8 women in the U.S. will develop breast cancer in her lifetime. China is not a country with a high incidence of breast cancer, but it should not be optimistic. In recent years, the growth rate of the incidence of breast cancer in my country is 1 to 2 percentage points higher than that of countries with a high incidence. According to the 2009 breast cancer incidence data released by the National Cancer Center and the Ministry of Health's Bureau of Disease Control and Prevention in 2012, the incidence of breast cancer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574G01N33/531
CPCG01N33/6803G01N33/531G01N33/57484
Inventor 王团老胡修永洪万进
Owner XIAMEN UNIV
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