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A technology of plant tissue culture and Gesneriaceae, which is applied in the field of plant tissue culture, can solve the problems of low multiplication coefficient of subculture culture, failure to reach 100% rooting rate, callus induction rate, etc., and achieve high-efficiency tissue culture The effect of rapid propagation system
Active Publication Date: 2016-11-16
四川立德种苗科技有限公司
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[0003] At present, there have been many reports on the tissue culture technology of Gesneria, but in many existing technologies, the differentiation rate of the first-generation cultured adventitious buds and the proliferation coefficient of the subculture culture are relatively low, and the rooting rate has not been able to reach 100%. %, as disclosed in the patent CN102144556A, in the method for the tissue culture and rapid propagation of the Yasuosaurus lettuce, its adventitious bud differentiation rate is only 31.7%, and the reproduction coefficient is only 4.6/60d, and the rooting rate is 90.7%; The propagation method of Brassica sativa, the multiplication factor of the subculture culture recorded in it is 3.6 times; CN105325301A discloses a method for the rap
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Embodiment 1
[0041] Embodiment 1: Gesneriaceae plant tissue culture method of the present invention
[0042] Explant sterilization:
[0043] Take the leaves and petioles of healthy Gesneria plants (Primula multi-scar, Wuxuan Primula, Yongfu Primula, Vein-Sparse Semicapsule, Medicinal Primula, Pig’s Ear) for research, After rinsing with tap water, move it into an ultra-clean workbench, soak it in 75% alcohol for 30-60s, wash it twice with sterile water, and then wash it with 0.1% HgCl 2 (add 2 drops of TWeen-20) solution for 5 minutes, wash with sterile water once; then use 0.1% HgCl 2 (Add 2 drops of TWeen-20) solution to treat once, leaves 2-3min, petiole 3-5min, wash 5-6 times with sterile water. Put the leaves or petioles on sterile filter paper to absorb water, cut the leaves into 0.5cm × 0.5cm pieces, and cut the petioles into 0.5-1.0cm long pieces for later use.
[0044] Callus induction and adventitious bud differentiation:
[0045] The leaves that have been sterilized and treat...
[0057] As can be seen from the results in Table 1, compared with the callus induction rate, adventitious bud differentiation rate, clustered bud proliferation coefficient and rooting rate of the existing patents, the present invention has achieved better results in all aspects, especially the clustered bud proliferation coefficient is Extremely significantly higher than the effects of current patented technologies.
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Abstract
The invention relates to the field of plant tissue culture and discloses a tissue culture method for gesneriaceae plants. The method comprises the following steps: taking an explant of a gesneriaceae plant and sterilizing; inoculating to a specific initial induction medium, performing dark culture and inducing callus, and then performing light culture and dividing into adventitious buds; inoculating the sprout tubers of the divided adventitious buds to the specific initial induction medium for performing enrichment culture, thereby obtaining available seedlings of multiple shoots; transplanting the available seedlings to a specific rooting culture medium for performing rooting culture, thereby acquiring complete aseptic seedlings. According to the invention, the specific initial induction medium, subculture medium and rooting culture medium are selected, a tissue culture technique is utilized and a new method is adopted for acquiring the aseptic seedlings of various gesneriaceae plants including primulina hance, Wuxuan primulina hance, Yongfu primulina hance, hemiboea cavaleriei var. paucinervis, officinal primulina hance, plantago asiatica, and the like, and a stable and effective tissue culture rapid propagation system is established.
Description
technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for plant tissue culture of Gesneriaceae. Background technique [0002] The Gesneriaceae (Gesneriaceae) plants are mostly perennial herbaceous plants with a wide variety. There are about 140 genera and more than 2,000 species in the world. There are 58 genera and 463 species in my country. Some species are resistant to drought, shade and humidity, some have high medicinal value, some have rich plant type, leaf shape and flower shape, beautiful flowers and leaves, high ornamental value, and have a certain indoor ornamental value. Potted flower development prospects; but most species have not yet entered the commercial development stage, and due to their own genetic defects and over-exploitation of wild resources, many Gesneriaceae plants with high application value are on the verge of sterilization. The use of tissue culture rapid propagation technology is conducive t...
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