Rice photoperiod-temperature-sensitive genic male sterility line hybridization fruit setting accelerant and preparation method and application thereof

A technology of photothermosensitive genic male sterile and preparation method, which is applied in the field of rice photothermosensitive genic male sterile line hybrid seeding promoter and its preparation field, can solve the problems of not promoting hybrid seeding, etc., and achieve improvement of seed setting rate and seed yield, The effect of reducing adverse effects and improving the vitality of the stigma

Active Publication Date: 2016-11-16
YANGZHOU UNIV
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no way to promote the hybridization of rice photothermosensitive genic male sterile lines (the fertilization of the ovary of the sterile line after the rice photothermosensitive genic male sterile line is crossed with the restorer line), especially to reduce the impact of high temperature on the rice photothermosensitive genic male sterile line. Chemical Control Methods of Harm in Hybridization

Method used

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  • Rice photoperiod-temperature-sensitive genic male sterility line hybridization fruit setting accelerant and preparation method and application thereof
  • Rice photoperiod-temperature-sensitive genic male sterility line hybridization fruit setting accelerant and preparation method and application thereof

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Embodiment Construction

[0013] (1) Material preparation

[0014] 1. Acetic acid (Acetic acid), molecular formula: C 2 h 4 o 2 , molecular weight, 60.05, grade: chemically pure, 500mL per bottle. According to the dosage per hectare 38.4 ~ 57.6mL, or every 667m 2 Dosage 2.56 ~ 3.84mL Calculate the total dosage.

[0015] 2. Ethanol (ethanol), chemical formula: C 2 h 5 OH, molecular weight, 46.07, grade: analytical grade, absolute ethanol, 500mL per bottle. According to the dosage per hectare 19.2 ~ 28.8mL, or every 667m 2 Dosage 1.28 ~ 1.92mL Calculate the total dosage.

[0016] 3. 24-Epicastasterone (24-Epicastasterone), molecular formula: C 28 h 46 o 6 , molecular weight, 480.68, purity: 92%, 500mg per bottle. According to the dosage per hectare 9.6 ~ 14.4mg, or every 667m 2 Dosage 0.64 ~ 0.96mg to calculate the total dosage.

[0017] 4. According to the ratio of 24-epibrassinolide:ethanol=1mg:2mL, dissolve 24-epibrassinolide in ethanol.

[0018] Both acetic acid and ethanol are sold in...

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Abstract

The invention provides a rice photoperiod-temperature-sensitive genic male sterility line hybridization fruit setting accelerant and a preparation method thereof. The accelerant is prepared from acetic acid, ethyl alcohol, 24-epibrassinolide and water. A solution is prepared in proportion and then sprayed in a heading period, therefore, the vitality of rice photoperiod-temperature-sensitive genic male sterility line pistil can be improved, water absorption germination of restoring line pollen grains on a photoperiod-temperature-sensitive genic male sterility line stigma and growth of a pollen tube are promoted, and the fruit setting rate and the seed yield of a pollinated ovary are increased. When high temperature occurs in the heading and flowering period, adverse influences of the high temperature on hybridization fruit setting and the seed yield of a rice photoperiod-temperature-sensitive genic male sterility line can be effectively reduced by spraying the rice photoperiod-temperature-sensitive genic male sterility line hybridization fruit setting accelerant solution, and therefore losses are reduced.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a rice photothermosensitive male sterile line hybrid seeding promoter and a preparation method thereof. The hybrid seed set in the present invention refers to the fertilization of the ovary of the sterile line after hybridization of the rice photothermosensitive genic male sterile line and the restorer line, and the seed set rate refers to the fertilization rate of the ovary. Background technique [0002] The two-line hybrid rice based on the photothermosensitive genic male sterile line of rice has the advantages of free combination, easy acquisition of strong heterosis combination, and low seed production cost. However, the seed setting rate (fertilization rate of ovary) and seed yield of rice photothermosensitive genic male sterile line and restorer line are generally low. Especially during the heading and flowering stage, when encountering high temperature, the seed set...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/22A01N37/02A01N31/02A01P21/00A01H1/02
CPCA01H1/02A01N31/02A01N37/02A01N43/22A01N2300/00
Inventor 杨建昌陈静王志琴
Owner YANGZHOU UNIV
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