Rice photoperiod-temperature-sensitive genic male sterility line hybridization fruit setting accelerant and preparation method and application thereof
A technology of photothermosensitive genic male sterile and preparation method, which is applied in the field of rice photothermosensitive genic male sterile line hybrid seeding promoter and its preparation field, can solve the problems of not promoting hybrid seeding, etc., and achieve improvement of seed setting rate and seed yield, The effect of reducing adverse effects and improving the vitality of the stigma
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[0013] (1) Material preparation
[0014] 1. Acetic acid (Acetic acid), molecular formula: C 2 h 4 o 2 , molecular weight, 60.05, grade: chemically pure, 500mL per bottle. According to the dosage per hectare 38.4 ~ 57.6mL, or every 667m 2 Dosage 2.56 ~ 3.84mL Calculate the total dosage.
[0015] 2. Ethanol (ethanol), chemical formula: C 2 h 5 OH, molecular weight, 46.07, grade: analytical grade, absolute ethanol, 500mL per bottle. According to the dosage per hectare 19.2 ~ 28.8mL, or every 667m 2 Dosage 1.28 ~ 1.92mL Calculate the total dosage.
[0016] 3. 24-Epicastasterone (24-Epicastasterone), molecular formula: C 28 h 46 o 6 , molecular weight, 480.68, purity: 92%, 500mg per bottle. According to the dosage per hectare 9.6 ~ 14.4mg, or every 667m 2 Dosage 0.64 ~ 0.96mg to calculate the total dosage.
[0017] 4. According to the ratio of 24-epibrassinolide:ethanol=1mg:2mL, dissolve 24-epibrassinolide in ethanol.
[0018] Both acetic acid and ethanol are sold in...
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