Real-time fluorescent PCR mixed micro-channel chip

Abstract

The invention provides a real-time fluorescent PCR mixed micro-channel chip. The chip comprises a sampling plug, a sealing film, a micro-channel mixed part and an injection part, wherein the sampling plug is embedded into the micro-channel mixed part; the micro-channel mixed part is injected with a sample and is sealed, and is used as a gas source inlet; the sealing film covers the upper surface of the micro-channel mixed part; enzyme, buffer solution and PCR probe liquid are preserved in the micro-channel mixed part, so that a sample can be mixed with the enzyme and buffer solution, is quantitatively controlled, and is driven to the injection part, and the PCR probe liquid is driven to the injection part; and the injection part is used for injecting the mixed solution and the PCR probe liquid into a PCR reaction tank below the injection part. The real-time fluorescent PCR mixed micro-channel chip is convenient to operate, can realize early mixing and probe injection of real-time fluorescent PCR operation as long as a sample is injected into the micro-channel chip to greatly improve the operation efficiency, reduce the operation flow and reduce the workload of personnel operation, and has a wide popularization meaning.

Description

technical field [0001] The invention relates to the field of medical equipment, in particular to a real-time fluorescent PCR mixed microfluidic chip. Background technique [0002] At present, the real-time fluorescent PCR method is mostly used for accounting detection. Conventional detection includes multiple sites. For each site, the operator needs to mix the sample extracted from the accounting with enzyme and buffer and quantitatively add it to 96 wells. One well of the plate, and then add the PCR probe to the well of the same 96-well plate. The whole process is handled manually. Especially for the detection of multiple sites, the operation is complicated and the probability of error is high. There is no easy way to realize it. . [0003] After retrieval, the publication number is CN1804043A, and the application number is CN200510011180.4 Chinese invention patent, which discloses a polymerase chain reaction chip microsystem, including a polymerase chain reaction (PCR) ch...

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Problems solved by technology

[0004] However, the above-mentioned patents have the following deficiencies: they cannot solve the problem of mixing and quantifying nucleic acid purification samples, buffers, and enzymes before polymerase chain reaction, nor can they solve the problem of mixing quantitative mixtures and probe reagents.

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