Herba Carpesii Abrotanoidis extract and preparation method and application thereof

The technology of Tianmingjing and extract is applied in the field of Tianmingjing extract and its preparation, which can solve the problems of human and animal poisoning, environmental pollution of chemical pesticides, unreasonable use of chemical pesticides, etc., and achieve the effect of delaying drug resistance.

Inactive Publication Date: 2016-11-23
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to people's unreasonable use of chemical pesticides and the inherent shortcomings of pesticides
As a result, the use

Method used

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  • Herba Carpesii Abrotanoidis extract and preparation method and application thereof
  • Herba Carpesii Abrotanoidis extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Preparation of Tianmingjing Antibacterial Active Extract:

[0022] Take 100kg of Tianming refined dry sample, add 70% ethanol for reflux extraction twice, add 800kg 70% ethanol for the first time, add 600kg 70% ethanol for the second time, filter, combine the extracts, reclaim the ethanol from the extracts and concentrate to Dilute extract with a relative density of 1.05, extract the thin extract with ethyl acetate to obtain an ethyl acetate phase, pass the ethyl acetate phase through D101 macroporous adsorption resin, and first elute with 30% ethanol until it is colorless. The % ethanol eluate was discarded, and then eluted with 85% ethanol until it was colorless, the 85% ethanol eluate was collected, the ethanol was recovered and concentrated into a thick paste with a relative density of 1.25, and dried in vacuum to obtain Tianmingjing Antibacterial extract (based on lactone, the mass fraction is not less than 60%).

Embodiment 2

[0023] Embodiment 2: the preparation of Tianmingjing bactericidal microemulsion:

[0024] Add 2 parts of Tianmingjing’s antibacterial and refined extract to the adjusted stirring reaction kettle, dissolve 1 part of the pesticide adjuvant Termul 5030 with 2 parts of ethyl acetate, stir at a high speed at a temperature of 40°C to 50°C, and add deionized 3 parts of water, stirred at constant temperature for 1 hour, and 40% Tianmingjing bactericidal microemulsion was obtained.

Embodiment 3

[0025] Embodiment 3: Determination of Antibacterial Activity of Tianming Fine Microemulsion

[0026] Dilute 0.02 mL of Tianming fine microemulsion prepared in Example 2 to 10 mL with melted PDA medium, mix and pour it into a petri dish to prepare a plate with bacteria. Test the pathogenic bacteria with plate culture in advance for 5-7 days, use a sterilized hole puncher to make a bacterium cake (diameter is 4mm) on the bacterium colony that has been cultivated, then buckle the bacterium cake upside down in the center of the mixed medicine flat plate, and repeat each treatment 3 times. Set an equal amount of sterilized water as a control. The inoculated strain plate was placed in a constant temperature incubator (25±1°C) for cultivation, and the colony diameter was measured for 72 hours by the cross method, and the inhibition rate was calculated according to the following formula. The results are shown in Table 1.

[0027] Colony growth diameter = colony diameter -4

[0028]...

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Abstract

The invention discloses a Herba Carpesii Abrotanoidis extract and a preparation method and application thereof. A Herba Carpesii Abrotanoidis bactericide is safe to the environment, active compounds in the Herba Carpesii Abrotanoidis bactericide are natural substances and derived from the nature and form an intrinsic energy and material cycle metabolic pathway in a long-term evolutionary process, the Herba Carpesii Abrotanoidis bactericide is free of residues when being applied to the environment or plants, so that biological enrichment is avoided; the Herba Carpesii Abrotanoidis bactericide is relatively safe to non-target organisms, different from conventional pesticides in phytopathogen action mechanism and less prone to causing drug resistance of pathogenic bacteria after use and also has a delaying effect on drug resistance of the pathogenic bacteria to conventional bactericides.

Description

technical field [0001] The invention relates to plant extracts and applications thereof, in particular to Tianmingjing extracts, preparation methods and applications thereof. Background technique [0002] Since the 1940s, chemical pesticides have dominated the pesticide market due to their variety, quick effect, convenient use, and low price. However, due to people's irrational use of chemical pesticides and the inherent shortcomings of pesticides. As a result, problems such as environmental pollution, human and animal poisoning, and pesticide residues have occurred in the use of chemical pesticides. With the improvement of people's quality of life and the enhancement of environmental awareness, the development of bio-pesticides that are safe for humans and animals, non-polluting, low-toxic, and low-residue has become a new hot spot in the field of pesticide research. As a part of biologically rational pesticides, the development and research of botanical pesticides has al...

Claims

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Application Information

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IPC IPC(8): A01N65/12A01N25/04A01P3/00
Inventor 胡林峰朱红霞杨晓迅张百重崔茂金王昊鹏朱英慧李鹏
Owner HENAN INST OF SCI & TECH
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