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A kind of bacterium and its application in producing hexadecanoic acid

A technology of hexadecanoic acid and dibasic acid, which is applied in the field of bacteria that efficiently produce DC16, and can solve problems such as no discovery

Active Publication Date: 2019-07-16
SHANDONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, biologically produced DC 16 Both use Candida tropicalis ( Candida cloacae ) to complete, has not yet found the use of bacteria to produce DC 16 report

Method used

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  • A kind of bacterium and its application in producing hexadecanoic acid
  • A kind of bacterium and its application in producing hexadecanoic acid
  • A kind of bacterium and its application in producing hexadecanoic acid

Examples

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Embodiment 1

[0022] 1. Bacterial enrichment

[0023] Take 10 g of oil-contaminated soil near the Sinopec gas station in Huangdao District, Qingdao, and put it into a beaker, then add 90 mL of deionized water to soak and stir, let it stand for 30 minutes, take 5 mL of the leaching solution and add it to 100 mL of liquid In the enrichment medium, shake culture (150 rpm / min) at 28 °C for 5 days to obtain the enrichment medium; the composition of the enrichment medium ( / L): 3.0 g of beef extract, 10.0 g of peptone, 5.0 g of NaCl, Adjust the pH to 7.6 with NaOH, and sterilize at 121 °C for 20 min before use;

[0024] 2. Screening and purification of n-hexadecane fermentation bacteria

[0025] Screening medium composition ( / L): K 2 HPO 4 1.5g, MgSO 4 ·7H 2 O 0.5g, NH 4 NO 3 1.5g, FeCl 3 0.025g, anhydrous CaCl 2 0.01g, n-hexadecane 1.5g, agar 20g, adjust pH to 7.0 with 2M NaOH, sterilize at 121°C for 20 min, pour plate while hot, and obtain solid plate medium after cooling, add agar ...

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Abstract

The present invention provides a new bacterium for Acinetobacter belongs to the technical field of microorganisms, at the same time, it provides its application in the production of hexadecanoic acid, the bacterium provided by the invention can produce hexadecanoic acid, and adopts the bacterium provided by the invention to produce hexadecanoic acid acid, avoiding the use of Candida tropicalis to produce DC in the prior art 16 Harm of bacterial strain, new bacterial fermentation production DC provided by the invention 16 The productive rate is also greater than the productive rate prepared by other biological methods in the prior art.

Description

technical field [0001] The present invention relates to the efficient production of DC 16 bacteria, while involved in their production of DC 16 The method specifically involves the use of culture method to produce DC from petroleum-contaminated soil. 16 The bacteria were enriched, screened, isolated and identified, and the DCs produced by their metabolism 16 Separation, purification and determination. Background technique [0002] DC 16 It is an important synthetic raw material, which can be used to synthesize muskone instead of natural musk, and is used to prepare various Chinese patent medicines. DC 16 It does not exist in nature, and it is difficult to synthesize by chemical methods. Therefore, the biological metabolism method has become the production DC 16 the most effective way. So far, biologically produced DC 16 Both use Candida tropicalis ( Candida cloacae ) to complete, has not yet found the use of bacteria to produce DC 16 reports. With Candida tropical...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/64C12R1/01
CPCC12P7/64C12N1/205C12R2001/01
Inventor 陆洪省冯晓寒刘亚樵于梦梦
Owner SHANDONG UNIV OF SCI & TECH