Light efficiency regulation gene hpe1 and its application
A technology for regulating genes and genes, which can be used in applications, genetic engineering, plant genetic improvement, etc., and can solve problems such as limited available targets.
Inactive Publication Date: 2019-07-26
SUN YAT SEN UNIV
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Nevertheless, currently available targets for effectively improving photosynthetic efficiency are still relatively limited, and systematic screening of available targets for improving photosynthetic efficiency is still a hot issue in the future.
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[0024] The inventors found through experiments that HPE1 The gene is located on chromosome 1 of Arabidopsis thaliana, and the gene locus number is LOC_AT1G70200 (TAIR accession number). Its full-length genome sequence is about 2104bp, including 4 exons and 3 introns. The full length of its cDNA is 1883 bp (SEQ ID NO: 2), encoding 538 amino acids.
[0025] HPE1 The encoded protein sequence is shown as SEQ ID NO: 3, which contains a chloroplast signal peptide (Chloroplast Transit Peptide, CTP) domain and an RNA recognition domain (RNA Recognition Motif, RRM).
[0026] The inventors confirmed by experiments that HPE1 Participating in the regulation of photosynthetic efficiency in Arabidopsis, it is an important functional gene that regulates plant photosynthetic efficiency. Using the forward genetics approach, two HPE1 The homozygous deletion mutant line found that HPE1 The functional loss of the gene can effectively optimize the light harvesting system, improve the efficie...
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The invention discloses a lighting effect regulation and control gene HPE1 and application thereof. An inventor screens one high-lighting-effect mutant HPE1 from an arabidopsis mutant library, clones it to a gene HPE1 with a code containing an RRM structure domain chloroplast protein through molecular identification and proves that the HPE1 participates in arabidopsis lighting effect regulation and control and is an important functional gene for regulating and controlling the photosynthetic efficiency of plants. By adopting a forward genetics method, homozygous deletion mutant strains of two HPE1 are obtained, it is found that function deficiency of the HPE1 gene can effectively optimize a light catching system, improves the efficiency of plant photosynthesis and increase organic matter accumulation and overall plant biomass. The HPE1 gene sequence (protein) has sequence conservation in other crops, it is indicated that the function of the HPE1 in other crops possibly has conservation, is can be predicted that the HPE1 gene is the important functional gene for regulating and controlling the photosynthetic efficiency of plants, and crops having high photosynthesis efficiency can be obtained by utilizing the gene.
Description
technical field [0001] The invention relates to a new gene and its application, in particular to a new light effect regulating gene and its application. Background technique [0002] As the global population continues to increase, food shortages have gradually become one of the world's problems, and it is imminent to increase crop yields (Food and Agriculture Organization of the United Nations, 2015). Photosynthesis is known as the greatest biochemical reaction on earth. It can use solar energy to convert inorganic matter into organic matter, completing the double conversion of matter and energy (Vinyard et al., 2013), and its efficiency directly determines crop yield (Long et al. ., 2006a). According to statistics, the light energy used by all plants on the earth through photosynthesis does not exceed 0.1% on average. The energy that is actually converted into various substances of crops in the solar energy is less than 5.0% of the solar energy that is converted into biom...
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IPC IPC(8): C12N15/29
CPCC07K14/415
Inventor 王宏斌靳红磊刘兵冯冬茹王金发
Owner SUN YAT SEN UNIV