Method for improving plant traits

A plant trait, plant technology, applied in the field of biology

Inactive Publication Date: 2013-07-10
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no one way that has been fully proven to effectively improve the light energy use efficiency of plants.

Method used

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  • Method for improving plant traits
  • Method for improving plant traits
  • Method for improving plant traits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0362] Example 1. LEAT protein uses light energy to catalyze the splitting of water

[0363] 1. LEAT protein catalyzes the reduction of 2,3,5-trimethyl-1,4-p-benzoquinone, an analogue of plastoquinone, by water under the action of light.

[0364] It has been reported that proteins such as GFP can act as electron donors to reduce small molecular compounds such as NAD in oxidation states. ＋ , potassium ferricyanide, and some proteins such as cytochrome C, and proteins with FAD as a prosthetic group (BogdanovAM et al., 2009, Nature Chemical Biology.5:459-461). The present inventors expressed recombinant proteins using purified Escherichia coli. Although pre-illuminated YFP and mCherry can be used as electron donors, one LEAT protein molecule can only provide one electron, and cannot provide electrons continuously under light ( figure 1 ). The photoreduction of quinone molecules catalyzed by LEAT proteins was analyzed by monitoring the decrease in the absorption of quinone mole...

Embodiment 2

[0372] Example 2. Comparison of the ability of LEAT proteins from different sources to catalyze water splitting to release oxygen under light

[0373] Nine fluorescent proteins and three non-fluorescent chromogenic proteins ( Figure 10 ). These 12 proteins belong to different branches such as A, B, C, and D on the evolutionary tree ( Figure 10 ). These 12 fluorescent proteins and non-fluorescent chromogenic proteins plus GFP series and dsRED series fluorescent proteins cover most of the currently reported fluorescent proteins and non-fluorescent chromogenic proteins (Alieva et al., 2008, Diversity and evolution of coarl fluorescent proteins.PLoS One3(7):e2680.doi:10.1371 / journal.pone.0002680), their molecular evolution pathways and amino acid homology are very different from the LEAT proteins of the GFP series and dsRED series ( Figure 11 A and 11B). Using Escherichia coli to express these proteins, it was found that although their fluorescence intensity, absorption spe...

Embodiment 3

[0382]Example 3, Functional research of LEAT protein mCherry transgenic plants

[0383] 1. The expression of mCherry protein in transgenic rape increased the net photosynthetic rate, promoted plant growth, and increased the biomass of transgenic plants.

[0384] After the mCherry transgenic rape is obtained, the mCherry transgenic rape is identified. The results of Southern blot identification of mCherry transgenic rapeseed are as follows Figure 13 B; Fluorescent observation in root cells of transgenic plants Figure 13 C; The results of RT-PCR and Western blot identification of transgenic plants (L1-L6) are as follows Figure 13 d. The expression and subcellular localization of mCherry protein were detected by immunoelectron microscopy. Figure 13 e. It can be seen that the plants L1-L6 are all mCherry positive transformed plants, and the mCherry protein is mainly expressed in the cytoplasm (Cy) and nucleus (N). The present invention uses the T2 generation mCherry tran...

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Abstract

The invention relates to a method for improving plant traits and discloses for the first time a novel plant improving method with improvement of light use efficiency of a plant as a purpose. According to the method, a light energy absorption and transduction protein (LEAT protein) is expressed in a plant, absorbed light energy interacts with related methylquinone derivatives, e.g., plastoquinone, in the plant to catalyze cracking of water and release oxygen, so light use efficiency of the plant is improved. The method provided by the invention can effectively expand use of light energy by the plant and increase photosynthetic efficiency and yield.

Description

technical field [0001] The invention belongs to the field of biotechnology; more specifically, the invention relates to a method for improving plant traits. Background technique [0002] Photosynthesis is the most important chemical reaction on earth. Through photosynthesis, plants convert light into a stable form of chemical energy that can be directly used by humans, and provide oxygen and food for human survival. However, in terms of the utilization efficiency of solar energy by plants, calculated from the entire growing season, the light energy that field crops can use is less than 1% of the solar energy received by crops on the earth's surface throughout the growing season. The use of light by plants mainly depends on the following aspects: First, the capture of light by plants. For higher plants, the light energy capture efficiency of leaves can generally reach more than 80%; the chlorophyll a and b of plants mainly absorb the red and blue parts of visible light for p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12N15/63C12N5/10C07K14/435A01H5/00A01H5/10
CPCC07K14/415C07K14/43504C12N9/90C12N15/8269C12Y401/01039C12N15/8209
Inventor 蔡伟明孙卫宁陈根云朱新广米华玲陈海莹
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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