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A rapid detection reagent for urine protein and its preparation method and application

A technology for detecting reagents and urine protein, which is applied in the field of medical detection, can solve the problems of missed detection of Zhou's protein and wrong judgment, and achieve the effects of high specificity, timely treatment, and strong sensitivity

Active Publication Date: 2018-08-28
ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is difficult for ordinary patients and their family members to achieve, or it is easy to cause misjudgment, and negative results may miss Zhou's protein and microalbumin

Method used

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  • A rapid detection reagent for urine protein and its preparation method and application
  • A rapid detection reagent for urine protein and its preparation method and application

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preparation example Construction

[0022] First, the present invention provides a method for preparing a urine protein rapid detection reagent, the method comprising preparing sodium polyacrylate and water according to 90-95:5-10 parts by weight, most preferably the weight ratio of the two is 92:8, and The total weight of the two is 100 parts to obtain polymer water-absorbing particles; configure a sodium dihydrogen phosphate buffer solution with a pH of 7.4±0.2, and dissolve the protein indicator in a sodium dihydrogen phosphate buffer solution with a pH of 7.4±0.2 , obtain the protein indicator buffer solution of 0.1-1.5g / L (its concentration is more preferably 0.1-0.5g / L), described protein indicator is the inorganic reagent that meets protein and can change color, whether protein exists, The indicator can be made to change color. Tetrabromophenol blue is preferred in this embodiment. For example, the amino group of the a-amino acid constituting the protein can be replaced with the hydroxyl group in the tetr...

Embodiment 1

[0027] The preparation method of the urinary protein rapid detection reagent described in the present embodiment comprises the following steps:

[0028] 1) Sodium polyacrylate and water are prepared according to 92:8 parts by weight, the total weight of the two is 100 parts, and polymer water-absorbing particles are obtained;

[0029] 2) Prepare a sodium dihydrogen phosphate buffer solution with a pH of 7.4, and dissolve the protein indicator (tetrabromophenol blue) in a sodium dihydrogen phosphate buffer solution with a pH of 7.4 to obtain a 0.5 g / L protein indicator buffer solution; The preparation of the damping fluid of above-mentioned PH7.4 is as follows: A liquid: 0.2mol / L sodium dihydrogen phosphate aqueous solution sodium dihydrogen phosphate (NaH 2 PO·H 2 0) 27.6g, dissolved in 1,000ml of distilled water; B solution: 0.2mol / L disodium hydrogen phosphate aqueous solution disodium hydrogen phosphate (Na 2 HPO4·7H 2 0) 53.6g, add distilled water to 1,000ml to complete...

Embodiment 2

[0032] The preparation method of the urinary protein rapid detection reagent described in the present embodiment comprises the following steps:

[0033] 1) Sodium polyacrylate and water are prepared according to 94:6 parts by weight, the total weight of the two is 100 parts, and polymer water-absorbing particles are obtained;

[0034] 2) Configure a sodium dihydrogen phosphate buffer solution with a pH of 7.4±0.1, and dissolve the protein indicator (tetrabromophenol blue) in a sodium dihydrogen phosphate buffer solution with a pH of 7.4 to obtain a protein indicator of 1-1.5g / L Buffer solution; The preparation of the buffer solution of described PH7.4 is as embodiment 1;

[0035] 3) Mix the polymer water-absorbing particles described in step 1) with the protein indicator buffer solution in a weight ratio of 6-8:94-92, the amount of the two is 100 parts by weight, so that the polymer water-absorbing particles swell to a diameter of The polymer water-absorbing beads with a diam...

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Abstract

The invention discloses a rapid urinary protein detection reagent and a preparation method and application thereof. The preparation method comprises the steps: preparing sodium polyacrylate and water according to the ratio (in parts by weight) of (90 to 95): (5 to 10), so as to obtain macromolecular water absorption granules, wherein the total parts (by weight) of sodium polyacrylate and water is 100 parts; dissolving a protein indicator into a buffer solution of sodium dihydrogen phosphate with the pH of 7.4+ / -0.2, so as to obtain a protein indicator buffer solution; mixing the macromolecular water absorption granules with the protein indicator buffer solution so as to swell the macromolecular water absorption granules into macromolecular water absorption drops with the diameter of 20mm+ / -2mm, thereby obtaining the rapid urinary protein detection reagent. The preparation method of the rapid urinary protein detection reagent, disclosed by the invention, is simple, and the obtained rapid urinary protein detection reagent has high specificity and high sensitivity, can be used for detecting trace albumin and has the advantages of rapidness and accuracy in detection.

Description

technical field [0001] The invention belongs to the field of medical detection, and in particular relates to a urine protein rapid detection reagent and a preparation method and application thereof. Background technique [0002] There are about 70 million nephropathy patients in my country, including nephrotic syndrome, and protein quantitative detection is an important treatment detection index. The determination of urine protein is the basis for the diagnosis of kidney disease, and the detection methods include qualitative and quantitative. Currently widely used qualitative methods for urine protein include sulfosalicylic acid method, heating acetic acid method, and dry chemical test strip method. Quantitative determination of urine protein is an indispensable inspection method for kidney disease, and the result obtained is: total urine protein. Currently commonly used methods include turbidimetric method, dye binding method, and chemical colorimetric method. However, m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N21/78
CPCG01N21/78G01N33/6827
Inventor 陈容平李双喜
Owner ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV