Method for restraining fruit postharvest diseases through induced resistance and adopted preparation

A technology for inducing resistance and post-harvest disease, applied in application, fruit and vegetable preservation, food preservation, etc., can solve the problems of chemical fungicides on human health and environmental safety hazards, pathogenic microorganism resistance, and affect the effect of disease control, etc. Achieve the effect of reducing postharvest disease, cost-effective, and easy to store

Active Publication Date: 2016-12-07
上海新溢生物科技工程研究中心(有限合伙)
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, the residues of chemical fungicides are likely to cause serious harm to human health and environmental safet

Method used

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Examples

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Embodiment 1

[0033] Example 1. Preparation for controlling postharvest disease of fruit

[0034] The Cryptococcus lorensis cells were inactivated in a high-pressure steam sterilizer (sterilized at 121° C. for 20 min) to obtain inactivated yeast cells.

[0035] Take 100 mg of inactivated yeast cells, suspend in 0.5 mL of 0.2 mol / L phosphate buffer solution (PBS, pH 8.0), and add 0.5 g of acid-washed glass beads (Model G8772 produced by Sigma, USA, particle size 425-600 μm), Grind and disrupt cells on an automatic sample grinder (frequency is 70Hz), 3min / cycle, process 5 cycles, centrifuge at 4000rpm and 4°C for 10min after crushing, wash the precipitate with sterile water until the supernatant is clarified, and finally centrifuge (4000rpm). , 4°C centrifugation for 10 min) to collect the precipitate, which is the cell wall of Cryptococcus lorens (referred to as yeast cell wall).

[0036] Take 50 g of Cryptococcus lorensis cell walls, add 1 L of water to prepare a cell wall suspension, and ...

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Abstract

The invention discloses a preparation for restraining fruit postharvest diseases through induced resistance. The preparation is prepared from cryptococcus laurentii cell walls and water and per liter comprises 4-6 g of cryptococcus laurentii cell walls. The invention further provides a method for restraining fruit postharvest diseases through induced resistance by adopting the preparation. Before fruits are boxed and stored, a first pretreatment way or a second pretreatment way is adopted. According to the first pretreatment way, the fruits are placed into the preparation to be soaked at first, drained off and placed into a container to be kept in a sealed state for 23-25 h; according to the second pretreatment way, the surfaces of the fruits are sprayed with the preparation before the fruits are picked, and then the fruits are placed into a container to be kept in a sealed state for 23-25 h; the pretreated fruits are taken out of the container and boxed. By the adoption of the method and the preparation, the fruit postharvest diseases can be effectively controlled on the premise of using no chemical bactericide.

Description

technical field [0001] The invention relates to the technical field of fruit postharvest disease prevention and control, and more particularly relates to a biological preservation technology for inhibiting fruit postharvest disease by inducing fruit resistance. Background technique [0002] The loss of fruits due to post-harvest rot is huge. According to statistics, more than 80 million tons of fruits and vegetables rot in my country every year, resulting in economic losses of 100 billion yuan. Infection by pathogenic fungi is the main cause of postharvest rot of fruits. Common pathogenic fungi are Penicillum spp., Botrytis spp. and Alternaria spp. [0003] At present, chemical fungicides are still the main measures to control postharvest diseases of fruits. Taking citrus as an example, the Chinese invention patent (application number 200910028802.2, citrus preservative) provides a variety of chemical fungicides that can control citrus diseases, including thiophanate-methyl...

Claims

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Application Information

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IPC IPC(8): A23B7/154
CPCA23B7/154
Inventor 余挺傅达
Owner 上海新溢生物科技工程研究中心(有限合伙)
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