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Application of honokiol nano particles of targeted liver sinusoidal endothelial cells in treatment of hepatic fibrosis

A technology of endothelial cells and liver fibrosis, applied in the direction of medical preparations of non-active ingredients, digestive system, active ingredients of hydroxyl compounds, etc., can solve problems such as few researches, and achieve the effect of accelerating clearance efficiency

Active Publication Date: 2016-12-07
BEIJING PROTEOME RES CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In terms of chronic liver injury research, there are not many studies on honokiol, but its correlation with chronic liver disease has also been reported

Method used

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  • Application of honokiol nano particles of targeted liver sinusoidal endothelial cells in treatment of hepatic fibrosis
  • Application of honokiol nano particles of targeted liver sinusoidal endothelial cells in treatment of hepatic fibrosis
  • Application of honokiol nano particles of targeted liver sinusoidal endothelial cells in treatment of hepatic fibrosis

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Experimental program
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preparation example Construction

[0069] The preparation method of honokiol nanoparticles refers to the following literature: Qing Xu, Yuexian Liu, Shishuai Su, Wei Li, Chunying Chen*, Yan Wu*.Anti-tumor activity of paclitaxel through dual-targeting carrier of cyclic RGD and transferrin conjugated hyperbranchedcopolymer nanoparticles, Biomaterials, 2012, 33, 1627-1639, the specific steps are as follows:

[0070] Accurately weigh 50 mg of PEG-PLGA (14.7 μmol, Kishitani Nano) into a round-bottomed flask containing 500 μL DMSO solution, stir and dissolve at room temperature, add equimolar peptide cRGD (Suzhou Qiangyao Biology) and stir for 2 h, and use thin-layer chromatography TLC analysis followed the reaction until the complete disappearance of cRGD. Evaporate the solvent under vacuum conditions, then redissolve with distilled water, and then dialyze with deionized water to remove small molecular substances, dialysis bag (molecular weight cut-off 3500), concentrate for use, and obtain concentrated PEG-cRGD, wh...

Embodiment 1

[0073] Embodiment 1, the application of Honokiol nanoparticles in the treatment of liver fibrosis

[0074] 1. The effect of Honokiol nanoparticles on the expression of related proteins in rat liver sinusoidal endothelial cells in the stage of chronic liver injury

[0075] Chronic liver injury can cause liver fibrosis. This part of the experiment is to prove that Honokiol regulates the normal physiological function of liver sinusoidal endothelial cells by increasing the ratio of p-Erk1 / 2 / p-Akt.

[0076] 1. Honokiol nanoparticles act on mice with liver fibrosis caused by chronic liver injury

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Abstract

The invention discloses application of honokiol nano particles of targeted liver sinusoidal endothelial cells in treatment of hepatic fibrosis. The Honokiol particles of the targeted liver sinustoidal endothelial cells have the following purposes of preparing products for treating hepatic fibrosis, preparing products for inhibiting hepatic fibrosis, preparing products for promoting or maintaining or restoring the liver sinusoidal endothelial cells to be in a differentiation state, preparing products for improving the liver recovery promoting function of the liver sinusoidal endothelial cells, preparing products for improving the expression of HGF protein and / or Wnt2 protein in the liver sinusoidal endothelial cells and preparing products for inhibiting hepatic fibrosis tuberculation or promoting hepatic fibrosis node removal. The cRGD marked PEG nano particles are used for packaging Honokiol for inhibiting hepatic fibrosis.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of honokiol nanoparticles targeting liver sinusoidal endothelial cells in the treatment of liver fibrosis. Background technique [0002] Honokiol is a kind of Magnoliaceae deciduous tree Magnolia officinalis extract. It has obvious and lasting central muscle relaxation, central nervous system inhibition, anti-inflammatory, anti-bacterial, anti-pathogenic microorganisms, anti-ulcer, anti-oxidation, anti-aging, anti-tumor, lowering cholesterol and other pharmacological effects. It can inhibit Akt phosphorylation, and can also promote ERK1 / 2 phosphorylation. In previous research reports, honokiol can inhibit the formation of arterial thrombus by protecting vascular endothelial cells. Further studies have shown that honokiol Phenol can also inhibit the apoptosis of vascular endothelial cells by promoting the generation of cycloprostaglandin. In addition, honokiol can reg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/34A61K31/05A61P1/16
CPCA61K9/5153A61K31/05
Inventor 姜颖贺福初劳远翔李严严魏汉东
Owner BEIJING PROTEOME RES CENT