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Detection kit for age-related macular degeneration choroidal polypoid vascular disease

A technique for vascular lesions and choroid is applied in the field of kits for detecting age-related macular degeneration choroidal polyp-like vascular lesions, which can solve the problems of irreversible related lesions, no very effective treatment method for PCV, etc., so as to reduce visual impairment and improve accuracy. Effect

Active Publication Date: 2019-12-13
SICHUAN PROVINCIAL PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no very effective treatment for PCV. The remission methods include PDT and intravitreal injection of anti-angiogenic drugs and combination therapy, but the associated lesions cannot be reversed.

Method used

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  • Detection kit for age-related macular degeneration choroidal polypoid vascular disease
  • Detection kit for age-related macular degeneration choroidal polypoid vascular disease
  • Detection kit for age-related macular degeneration choroidal polypoid vascular disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Correlation between FGD6 gene rs77466370 site, ARMS2 / HTRA1 gene rs10490924 site, CFH gene rs800292 site, CFH gene rs1065489 site and age-related macular degeneration choroidal polypoid vasculopathy (polypoidal choroidal vasculopathy, PCV)

[0027] 1. Sample collection and genomic DNA extraction

[0028] The samples were collected from the Sichuan Provincial People's Hospital.

[0029] Genomic DNA was prepared from human peripheral blood according to the following method. In the presence of anticoagulant EDTA, 5 ml of human peripheral blood collected was centrifuged at 3000 rpm for 30 minutes to remove serum. A 0.2% NaCl solution was then added to bring the total volume to 50 ml. The solution was shaken gently 5-6 times and allowed to sit on ice for 15 minutes. Thereafter, the precipitate was collected by centrifugation at 3000 rpm for 30 minutes. With 0.2% NaCl solution, further washing was carried out in a similar manner as before. To the precipitate thu...

Embodiment 2

[0067] Embodiment 2 Detection kit of the present invention

[0068] All components, content and method of use in the kit of the present invention are as follows:

[0069] PCR amplification reagent (50 servings):

[0070]

[0071]

[0072] Sanpshot Typing Detection Reagent (50 copies)

[0073]

[0074]

[0075] Standard DNA sample:

[0076]

[0077] Instructions:

[0078] 1) DNA extraction

[0079] Take 2ml of whole blood (EDTA anticoagulated) from the patient, and extract its genomic DNA.

[0080] 2) PCR amplification of the DNA fragment containing the detected SNP site by PCR amplification (20 μl system):

[0081]

[0082] Reaction conditions:

[0083]

[0084] PCR product detection:

[0085] Use 2% agarose gel electrophoresis to detect the PCR product, observe the effect of the PCR reaction, and determine the amount of it added as a template in subsequent reactions.

[0086] 3) Sanpshot typing detection

[0087] The first step: PCR product purif...

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Abstract

The present invention discloses a kit for detecting choroid polypoid vascular disease. The kit comprises an optional reagent for detecting the variation of an rs77466370 site of an FGD6 gene. The kit for detecting choroid polypoid vascular disease can be used for early monitoring and detection of PCV high-risk groups, application of early prevention and treatment, and reduction of visual impairment.

Description

technical field [0001] The invention relates to a kit for detecting senile macular degeneration choroidal polypoid vascular disease, in particular a kit for detecting rs77466370 site of FGD6 gene related to senile macular degeneration choroidal polypoid vascular disease. Background technique [0002] Polypoidal choroidal vasculopathy (PCV), also known as choroidal polypoid vascular disease, is a fundus lesion typically characterized by abnormally branched choroidal vascular network and polypoid dilation of peripheral vessels. The fundus manifestations of PCV are similar to those of exudative age-related macular degeneration (AMD), but its indocyanine green angiography (ICGA) findings are significantly different from those of exudative age-related macular degeneration (AMD). the difference. [0003] PCV was first presented by Yannuzzi at a macular meeting in 1982 and described it as a distinctive hemorrhagic and / or serous retinal pigment epithelial and neuroepithelial detach...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 杨正林黄璐琳张候兵
Owner SICHUAN PROVINCIAL PEOPLES HOSPITAL
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