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Active preservation method of stem cell factor for beauty and skin care

A stem cell factor, beauty and skin care technology, applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc. The problem of short active storage time, etc., can achieve the effect of removing senescent apoptotic cells, prolonging active storage period, and good use effect.

Active Publication Date: 2019-04-30
GENESIS STEMCELL REGENERATIVE MEDICINE ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, in the state of cream or water emulsion of traditional beauty and skin care, the active preservation time of stem cell growth factors is very short, and the effect of beauty and skin care and anti-aging cannot be achieved, which greatly limits the role of stem cell active factors in beauty and skin care.

Method used

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  • Active preservation method of stem cell factor for beauty and skin care
  • Active preservation method of stem cell factor for beauty and skin care
  • Active preservation method of stem cell factor for beauty and skin care

Examples

Experimental program
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Effect test

Embodiment 1

[0033] The active preservation method of the stem cell factor used for beauty and skin care includes the selection of raw materials, the preparation of liposomes and the freeze-drying process to complete the active preservation of the stem cell factor for beauty and skin care; the specific steps are as follows:

[0034] (1) Selection of stem cell active factors: choose stem cell active factors with a purity greater than or equal to 99%;

[0035] (2) Preparation of liposomes: including selection of hydration solvent, determination of drug-to-lipid ratio, and ultrasonic coating process. The specific steps are as follows:

[0036] a. Selection of hydration solvent: choose PBS solvent with pH of 7.4, secondary water solvent with pH of 7.0, D-Hanks with pH of 7.4, D-Hanks with pH of 9.5, Nacl with a concentration of 0.9%, and a concentration of 5 One of the glucose in %;

[0037] b. Determination of drug-to-lipid ratio: the mass ratio of stem cell active factor to liposome is 1:2;...

Embodiment 2

[0041] The active preservation method of the stem cell factor used for beauty and skin care includes the selection of raw materials, the preparation of liposomes and the freeze-drying process to complete the active preservation of the stem cell factor for beauty and skin care; the specific steps are as follows:

[0042] (1) Selection of stem cell active factors: choose stem cell active factors with a purity greater than or equal to 99%;

[0043] (2) Preparation of liposomes: including selection of hydration solvent, determination of drug-to-lipid ratio, and ultrasonic coating process. The specific steps are as follows:

[0044] a. Selection of hydration solvent: choose PBS solvent with pH of 7.4, secondary water solvent with pH of 7.0, D-Hanks with pH of 7.4, D-Hanks with pH of 9.5, Nacl with a concentration of 0.9%, and a concentration of 5 One of the glucose in %;

[0045] b. Determination of drug-to-lipid ratio: the mass ratio of stem cell active factor to liposome is 1:1;...

Embodiment 3

[0049]The active preservation method of the stem cell factor used for beauty and skin care includes the selection of raw materials, the preparation of liposomes and the freeze-drying process to complete the active preservation of the stem cell factor for beauty and skin care; the specific steps are as follows:

[0050] (1) Selection of stem cell active factors: choose stem cell active factors with a purity greater than or equal to 99%;

[0051] (2) Preparation of liposomes: including selection of hydration solvent, determination of drug-to-lipid ratio, and ultrasonic coating process. The specific steps are as follows:

[0052] a. Selection of hydration solvent: choose PBS solvent with pH of 7.4, secondary water solvent with pH of 7.0, D-Hanks with pH of 7.4, D-Hanks with pH of 9.5, Nacl with a concentration of 0.9%, and a concentration of 5 One of the glucose in %;

[0053] b. Determination of drug-to-lipid ratio: the mass ratio of stem cell active factor to liposome is 1:1; ...

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Abstract

The invention discloses an active preservation method of stem cell factors used for cosmetic skincare. The active preservation method comprises the raw material selection, lipidosome preparation and freeze-drying process treatment, and thus the active preservation for the stem cell factors used for cosmetic skincare is completed. The active preservation method comprises the following specific steps: selecting the stem cell active factors, namely, selecting the stem cell active factors with the purity being greater than or equal to 99%; preparing lipidosome, namely, selecting a hydrated solvent, determining the drug-lipid ratio, and carrying out the ultrasonic laminating process treatment; and carrying out the freeze-drying process treatment, namely, dissolving the prepared lipidosome in a cell culture supernate at a certain concentration, adding with an excipient with a certain ratio, and carrying out vacuumizing in a freeze dryer so that the liquid sublimates, and thus powdery substance is formed. According to the active preservation method, the lipidosome preparation and the freeze-drying technology are adopted, so that the active preservation period of the stem cell active factors under the liquid state is effectively prolonged; the stem cell active factors are applied to the field of cosmetics, so that the efficacies of biological cosmetics are achieved, and the efficacies directly act on the cellular layer of the skin, so that the use effects are excellent.

Description

technical field [0001] The present invention relates to the field of method for preserving the activity of stem cell factor, in particular to a method for [0002] Active preservation method of stem cell factor for beauty and skin care. Background technique [0003] Stem cells are a type of pluripotent cells with self-replicating ability. Under certain conditions, it can differentiate into a variety of functional cells. Stem cells are divided into embryonic stem cells and adult stem cells according to their developmental stage. According to the developmental potential of stem cells, they are divided into three categories: totipotent stem cells, pluripotent stem cells and unipotent stem cells. Stem cells are the cells of origin. Stem cells have the ability to self-renew and replicate, and can produce highly differentiated functional cells. [0004] The aging of the human body and the appearance of wrinkles are essentially the aging and reduction of cells. The senescence...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/64A61Q19/00A61Q19/08
CPCA61K8/64A61Q19/00A61Q19/08
Inventor 殷鉴强刘定生杨敏敏
Owner GENESIS STEMCELL REGENERATIVE MEDICINE ENG CO LTD