Production method and application of human placenta decidua mesenchymal stem cell secreted factor freeze-dried powder
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A technology of secreting factors and freeze-dried powder, applied in the biological field, can solve the problem of insufficient nutritional value of Camellia oleifera seed oil, and achieve the effects of extending the shelf life, facilitating transportation, and avoiding irritation.
Pending Publication Date: 2020-10-27
江苏中衍生科细胞技术研究院有限公司
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[0004] The purpose of the present invention is to provide a preparation method and application of freeze-dried powder of human placental decidua stem cell secreted factor, so as to solve the problem that the nutritional value of Camellia oleifera seed oil currently on the market is not high enough in the above-mentioned background technology
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Embodiment 1
[0022] A preparation method of freeze-dried powder of human placental decidua interstem cell secreted factor, comprising the following steps:
[0023] Step 1: Take a full-term healthy newborn placenta, put it in a storage and transportation box containing a sterile preservation solution, and wait for separation and preparation;
[0024] Step 2: Remove the amniotic membrane, cut off all the fetal membranes, place them in a petri dish, wash them twice with 0.9% sodium chloride injection, until there is no obvious blood, separate the decidua tissue from the fetal membranes, and separate the decidua The tissue was collected in a centrifuge tube, added with an appropriate amount of 0.9% sodium chloride injection, centrifuged and washed 1-2 times. Discard the supernatant, cut into 1mm 3 the size of the tissue block;
[0025] Step 3: add collagenase to digest for 1.5h;
[0026] Step 4: Digestion Use the blood transfusion set filter to filter the digested mixture immediately, and t...
Embodiment 2
[0037] A preparation method of freeze-dried powder of human placental decidua interstem cell secreted factor, comprising the following steps:
[0038] Step 1: Take a full-term healthy newborn placenta, put it in a storage and transportation box containing a sterile preservation solution, and wait for separation and preparation;
[0039] Step 2: Remove the amniotic membrane, cut off all the fetal membranes, place them in a petri dish, wash them with 0.9% sodium chloride injection for 2-3 times, until there is no obvious blood, separate the decidua tissue from the fetal membranes, and separate the The decidua tissue was collected in a centrifuge tube, added an appropriate amount of 0.9% sodium chloride injection, and washed by centrifugation twice. Discard the supernatant, cut into 4mm 3 the size of the tissue block;
[0040] Step 3: add collagenase to digest for 1.5h;
[0041] Step 4: Digestion Use the filter of the blood transfusion set to filter the digested mixture immedi...
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Abstract
The invention discloses a production method and application of human placenta decidua mesenchymal stem cell secreted factor freeze-dried powder. The production method of the human placenta decidua mesenchymal stem cell secreted factor freeze-dried powder comprises the steps of separation and culture of placenta decidua stem cells, collection of placenta decidua mesenchymal stem cell supernate, purification of mesenchymal stem cell factors, and production of the mesenchymal stem cell factor freeze-dried powder. According to the production method of the human placenta decidua mesenchymal stem cell secreted factor freeze-dried powder, the placenta decidua mesenchymal stem cells are cultured by adopting a serum-free culture system, so that stimulation of components in FBS to skin is avoided; moreover, the mesenchymal stem cells are cultured under a low oxygen condition, so that secretion of a large number of the cell factors of the cells is stimulated; through a low-temperature freeze-drying technology, a product can be stored at a normal temperature, transportation is facilitated, and the storage life of the activity of the secreted factors is prolonged; and the obtained placenta decidua mesenchymal stem cell secreted factor freeze-dried powder can achieve multiple kinds of efficacy of skin wrinkle prevention, whitening, oxidation resistance, acne mark fading and the like by reanimating aged cells.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method and application of a freeze-dried powder of human placental decidua interstem cell secreted factor. Background technique [0002] Mesenchymal stem cells (MSC, mesenchymal stem cells) are important members of the stem cell family, derived from the mesoderm in the early stage of development, and belong to pluripotent stem cells. MSCs have multi-directional differentiation potential, promote the homing of hematopoietic stem cells, and repair damage or disease Functions of tissues and organs, immune regulation and self-replication. MSC was initially found in the bone marrow. Compared with bone marrow mesenchymal stem cells, placental decidua mesenchymal stem cells have the advantages of sufficient source, low immunogenicity, low virus contamination rate, and no social and ethical disputes, making it unique. Better application prospects. [0003] With the deepening ...
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