Production method and application of human placenta decidua mesenchymal stem cell secreted factor freeze-dried powder
A technology of secreting factors and freeze-dried powder, applied in the biological field, can solve the problem of insufficient nutritional value of Camellia oleifera seed oil, and achieve the effects of extending the shelf life, facilitating transportation, and avoiding irritation.
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Embodiment 1
[0022] A preparation method of freeze-dried powder of human placental decidua interstem cell secreted factor, comprising the following steps:
[0023] Step 1: Take a full-term healthy newborn placenta, put it in a storage and transportation box containing a sterile preservation solution, and wait for separation and preparation;
[0024] Step 2: Remove the amniotic membrane, cut off all the fetal membranes, place them in a petri dish, wash them twice with 0.9% sodium chloride injection, until there is no obvious blood, separate the decidua tissue from the fetal membranes, and separate the decidua The tissue was collected in a centrifuge tube, added with an appropriate amount of 0.9% sodium chloride injection, centrifuged and washed 1-2 times. Discard the supernatant, cut into 1mm 3 the size of the tissue block;
[0025] Step 3: add collagenase to digest for 1.5h;
[0026] Step 4: Digestion Use the blood transfusion set filter to filter the digested mixture immediately, and t...
Embodiment 2
[0037] A preparation method of freeze-dried powder of human placental decidua interstem cell secreted factor, comprising the following steps:
[0038] Step 1: Take a full-term healthy newborn placenta, put it in a storage and transportation box containing a sterile preservation solution, and wait for separation and preparation;
[0039] Step 2: Remove the amniotic membrane, cut off all the fetal membranes, place them in a petri dish, wash them with 0.9% sodium chloride injection for 2-3 times, until there is no obvious blood, separate the decidua tissue from the fetal membranes, and separate the The decidua tissue was collected in a centrifuge tube, added an appropriate amount of 0.9% sodium chloride injection, and washed by centrifugation twice. Discard the supernatant, cut into 4mm 3 the size of the tissue block;
[0040] Step 3: add collagenase to digest for 1.5h;
[0041] Step 4: Digestion Use the filter of the blood transfusion set to filter the digested mixture immedi...
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