Pig placentin production method
A production method, the technology of pig placenta, which is applied in the fields of application, food preparation, food science, etc., can solve the problems that the production method of pig placenta has not been reported, and achieve the effects of prolonging the shelf life of activity, being convenient to use, and improving fluidity
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Embodiment 1
[0028] Take fresh pig embryos (including placenta), wash them with pure water, and use an electric mixer to crush the embryos and placenta into a paste (porridge). Pass the sample through a 60-mesh sieve and collect the filtered sample. Add antioxidants (tea polyphenols (Victor powder) 0.02%, tertiary butyl hydroquinone TBHQ 0.01%) and lyoprotectants (sodium alginate 0.5%, Tween-80 0.5%, mannitol 4%, Alanine 1%, Glycine 1.5%), followed by low temperature and high pressure homogenization. Homogenization conditions: pressure 40Kg / cm 2 , the processing speed is 40 L / hr, the homogenate volume is controlled at 1 L each time, the homogenate time is 5 minutes (depending on the homogenate flow rate and sample volume), and the temperature is controlled at 0°C. The homogenized samples were divided into 400mL bottles in centrifuge cups, centrifuged at 10,000 rpm for 20 minutes, and the temperature was controlled at 0°C to 4°C. After centrifugation, take the supernatant and go through ...
Embodiment 2
[0031] Take fresh pig embryos (including placenta), wash them with pure water, add crushed ice made of pure water, and use an electric mixer to crush the embryos and placenta into a paste (porridge). Pass the sample through a 60-mesh sieve and collect the filtered sample. Add antioxidants (tea polyphenols (Victor powder) 0.02%, tertiary butyl hydroquinone TBHQ 0.005%) and lyoprotectants (sodium alginate 0.1%, Tween-80 0.01%, mannitol 2%, Alanine 2%, glycine 1%), add crushed ice made of citrate buffer, and carry out low temperature and high pressure homogenization. Homogenization conditions: pressure 60Kg / cm 2 , the processing speed is 60L / hr, the homogenate volume is controlled at 5L each time, the homogenate time is 10 minutes (depending on the homogenate flow rate and sample volume), and the temperature is controlled at 4°C. The homogenized samples were divided into 400mL bottles in centrifuge cups, centrifuged at 10,000 rpm for 20 minutes, and the temperature was controll...
Embodiment 3
[0033] The conventional animal embryo placenta extraction process (the finished product is freeze-dried and pulverized fine powder containing insoluble matter) and the process of the present invention are used to produce 1 kg of pig embryos, and the recovery rate and activity of active ingredients are compared. Porcine placenta activity was detected by lymphocyte rosette activity. The detection method is as follows:
[0034] Separate healthy human peripheral blood mononuclear cells with lymphatic separation medium according to conventional methods, detect cell viability (should be >95%) with 1% trypan blue staining, and count cells. Adjust the cell number to 2 x 10 with calf serum 6 / mL. 200 μL of the cell solution was taken into test tubes, and divided into the conventional animal embryo placenta extraction process control group and the soluble freeze-dried powder group, wherein the conventional animal embryo placenta extraction process control group was added to the sample...
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