Polymerase chain reaction machine and temperature control system thereof

Abstract

The invention relates to the technical field of polymerase chain reaction, specifically to a polymerase chain reaction machine and a temperature control system thereof. The system comprises multiple heating modules for temperature regulation of different areas of a porous reaction plate, thermal insulating layers which are arranged between different areas of the porous reaction plate and used for isolating heat exchange between different areas of the porous reaction plate, and a controller which is respectively connected to the multiple heating modules and used for controlling the multiple heating modules to respectively regulate temperature in different areas of the porous reaction plate. The porous reaction plate is divided into multiple areas beforehand to be in one-to-one correspondence with the multiple heating modules. By adding the thermal insulating layers between different areas of the porous reaction plate so as to isolate heat exchange between the different areas, thermal interference between the different areas of the porous reaction plate during temperature control is reduced so as to raise temperature control precision.

Description

technical field [0001] The invention relates to the technical field of polymerase chain reaction, in particular to a polymerase chain reaction instrument and a temperature control system thereof. Background technique [0002] Polymerase Chain Reaction (Polymerase Chain Reaction, referred to as PCR) is a molecular biology technique used to amplify specific DNA fragments. It can be regarded as special DNA replication outside the body. The biggest feature of PCR is that Can greatly increase the trace amount of DNA. Currently, the equipment used to perform the polymerase chain reaction is called a PCR machine. At present, there are many types of PCR instruments on the market, mainly divided into ordinary PCR instruments, gradient PCR instruments, in-situ PCR instruments, real-time fluorescent quantitative PCR instruments, etc. [0003] PCR is composed of three basic reaction steps: denaturation-annealing-extension. The PCR instrument controls the sample to reach different temp...

AI Technical Summary

Problems solved by technology

However, in the existing thermal cyclers, thermal interference usually exists between adjacent areas in this partition temperature control scheme, which makes the temperature control accuracy of different partitions low.

When users use it, in order to avoid mutual interference between different areas, they often need to limit the temperature difference between adjacent areas, and the use limitations are relatively large

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