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Method for increasing germination rate of Hypericum ascyron seeds

A technology of germination rate and yellow crabapple, which is applied in the fields of seed and rhizome treatment, botany equipment and methods, preservation of human or animal body, etc., can solve the problem of failure to effectively break the dormancy of yellow crabapple seeds, the germination rate is only 28%, Problems such as artificial breeding are difficult to achieve the effects of easy manual operation, improved germination rate, and low cost

Active Publication Date: 2017-01-04
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the study of seed germination characteristics, although it has been found that gibberellin treatment can increase the germination rate of Begonia chinensis seeds, the effect is not good, and the germination rate is only 28%, which cannot effectively break the dormancy of Begonia chinensis seeds and solve the problem of artificial breeding

Method used

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  • Method for increasing germination rate of Hypericum ascyron seeds

Examples

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Effect test

Embodiment 1

[0027] Collect the ripe yellow crabapple fruits from September to October, remove the husks after natural drying in the shade, remove impurities by winnowing, and select the clean seeds. Soak the seeds with a sodium hypochlorite solution with a mass concentration of 1% for 15 minutes for disinfection, then rinse with clean water and dry the water on the surface of the seeds. Soak the sterilized seeds in 0.1mM gibberellin GA 3 In the solution, remove the seeds after 24 hours, rinse them with clean water, place the seeds on a glass petri dish with two layers of moist filter paper, seal with plastic parafilm, store in low temperature at 4℃ for 3 weeks, wet During the process, water was added to the petri dish every 4 days to keep the filter paper moist. The filter paper and petri dishes are sterilized by high temperature and high pressure at 120°C for 20 minutes before use. After wet storage, the petri dish was taken out and placed in an incubator at 15°C with a light-dark cycle ...

Embodiment 2

[0029] Collect the ripe yellow crabapple fruits from September to October, remove the husks after natural drying in the shade, remove impurities by winnowing, and select the clean seeds. Soak the seeds with a sodium hypochlorite solution with a mass concentration of 1% for 20 minutes, then rinse them with clean water and dry the water on the surface of the seeds. Soak the sterilized seeds in 0.1mM GA 3 In the solution, remove the seeds after 24 hours, rinse them with water, place the seeds on a glass petri dish with two layers of moist filter paper, seal with a parafilm, store at low temperature at 2°C for 3 weeks, wet storage process Add water to the petri dish every 3 days to keep the filter paper moist. The filter paper and petri dishes are sterilized by high temperature and high pressure at 120°C for 20 minutes before use. After wet storage, the petri dish was taken out and placed in an incubator at 15°C with a light-dark cycle of 12h / 12h for germination for 28 days.

Embodiment 3

[0031] Collect the ripe yellow crabapple fruits from September to October, remove the husks after natural drying in the shade, remove impurities by winnowing, and select the clean seeds. Soak the seeds with a sodium hypochlorite solution with a mass concentration of 1% for 18 minutes, rinse them with clean water, and dry the surface water of the seeds. Soak the sterilized seeds in 0.2mM GA 3 In the solution, take out the seeds after 24 hours, rinse them with clean water, place the seeds on a glass petri dish with two layers of moist filter paper, seal with a parafilm, and store at 4℃ for 4 weeks at low temperature. The process of wet storage Add water to the petri dish every 4 days to keep the filter paper moist. The filter paper and petri dishes used were sterilized by high temperature and high pressure at 120°C for 30 minutes before use. After wet storage, the petri dish was taken out and placed in an incubator at 15°C with a light-dark cycle of 12h / 12h for germination for 2...

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Abstract

The invention discloses a method for increasing the germination rate of Hypericum ascyron seeds. The method comprises the following steps: preparing mature Hypericum ascyron fruits, naturally drying in the shade, removing shells, and removing impurities by virtue of an air blast method, so as to pick out pure seeds; soaking the seeds in a 1% sodium hypochlorite solution for 15-20 minutes, flushing the seeds with clean water, and airing the surface moisture of the seeds; soaking the aired seeds in a 0.1mM-0.3mM gibberellins solution for 20-24 hours, taking out the seeds, flushing the seeds with clean water, and carrying out wet preservation on the seeds at 2-5 DEG C for 3-4 weeks; and after the wet preservation, carrying out germination accelerating on the seeds in an incubator, wherein the temperature is 15 DEG C, the light-dark period is that 12-hour illumination and 12-hour dark are alternate, and the culturing time is 25-30 days. By virtue of the method, the germination rate of the Hypericum ascyron seeds can be substantially increased, the artificial breeding difficulty is reduced, and the large-scale planting of Hypericum ascyron is realized.

Description

Technical field [0001] The invention relates to the field of plant cultivation. More specifically, the present invention relates to a method for improving the germination rate of yellow crabapple seeds with steam. Background technique [0002] Yellow Begonia (Hypericum ascyron L.) is a perennial herb of the genus Hypericum in the Garciniaceae family. It is born in hillside forests, forest edges, shrubs, grasses or meadows, streams and riparian wetlands. Huang Haitang is one of the commonly used Chinese medicinal materials in my country. The above-ground part is used for medicinal purposes. It has the effects of calming liver, hemostasis, sepsis, reducing swelling, and anti-inflammatory and analgesic effects. Rheumatism, dysentery, and irregular menstruation. In addition, the yellow begonia flowers are numerous and large, and the color is gorgeous, which is extremely valuable in ornamental applications. Huanghaitang has many years of cultivation history, but its production has p...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01N59/20A01N59/16A01N61/00A01N43/90A01N43/12A01P21/00
CPCA01C1/00A01N43/12A01N43/90A01N59/16A01N59/20A01N61/00A01N2300/00
Inventor 潘春柳黄燕芬朱艳霞董青松缪剑华
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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