Dendrobium officinale strong plantlet and rootage culture medium and preparation method thereof

Abstract

The invention belongs to the technical field of dendrobium officinale planting, and particularly provides a dendrobium officinale strong plantlet and rootage culture medium and a preparation method thereof. The culture medium is formed by mixing a mother liquor I, a mother liquor II, a mother liquor III, a mother liquor IV, a mother liquor V, a mother liquor VI and a mother liquor VII with the volume percentage of 7-10:2-3:2-3:8-10:0.05-0.1:0.05-0.1:0.2-0.8. According to the invention, polyamine, abundant 481, Rui toxenzyme, dithane staneless, cinnamic acid and bi-sulfenyl propanol are added to promote growth of branches and leaves and root system, stimulate the rapid growth of seedlings, form sturdy rootstock, increase chlorophyll content, and enhance the photosynthetic capacity; the problems of brown stain, seedling yellowing and ageing and leaf abscission occurring in the process of tissue culture of dendrobium officinale can be improved; the problem of germ infection of plants can be improved; and the cold resistant and drought resistant capabilities of the plants can be improved.

Description

technical field [0001] The invention belongs to the technical field of dendrobium candidum planting, and in particular relates to a culture medium for rooting and strengthening seedlings of dendrobium candidum and a preparation method thereof. Background technique [0002] Dendrobium candidum is a perennial herbaceous plant of the family Dendrobium, mainly distributed in tropical and subtropical virgin forests and similar warm and humid areas, often attached to limestone or trees of bryophytes. Dendrobium candidum is a commonly used precious traditional Chinese medicine. It has the effects of nourishing yin and clearing heat, promoting body fluid and benefiting stomach, moistening lung and relieving cough, moisturizing throat and improving eyesight, and prolonging life. It is also rich in polysaccharides, alkaloids, total phenols and other biologically active substances. and antioxidant effects. However, due to the harsh growth environment of Dendrobium candidum, low natura...

AI Technical Summary

Problems solved by technology

However, due to the harsh growth environment of Dendrobium candidum, low natural yield and long-term over-excavation by people, wild resources are decreasing day by day; due to the fact that natural sources of Dendrobium candidum are becoming less and less, plant tissue culture has become a medicinal plant in recent years. The main method of reproduction and large-scale production of its active ingredients. There are many kinds of media for the tissue culture of Dendrobium candidum in the market, and the content of the ingredients is not the same, but the effect of these media on the rooting stage of Dendrobium candidum is not the same. Ideal, the stem is not strong enough, the root system is not enough, the transplanting survival rate is low, the growth rate is slow, etc., so that the quality of the seedlings is greatly reduced, which is not conducive to the market sales of Dendrobium candidum. For example, Chinese patent CN201510375351.5 improves the output, quality, and Biotransformation rate and transplanting survival rate under suitable environment, but failed to solve the problems of plant yellowing and senescence, leaf shedding, cold resistance and drought resistance, resistance, etc. change problem