Phytoextraction composition used for inhibiting tyrosinase and reducing or preventing melanogenesis and pharmaceuticals and application thereof

A technology of melanin and composition, applied in plant extracts of resveratrol, medicines for prevention and treatment of lightening skin color and melanin precipitation, prevention and treatment of lightening skin color and melanin precipitation, can solve the problem of whitening effect not as expected To reduce or prevent melanin production, skin tone or spot removal, and inhibit enzyme activity

Inactive Publication Date: 2017-01-11
CALIWAY BIOPHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In addition, in the past, when developing ingredients or products related to whitening applications, most experiments first added drugs, then gave α-MSH to stimulate melanin production, and then compared the melanin inhibition effect of the drug-added group; or only conducted in vitro test tubes. reaction, directly mix the drug and tyrosine, and then add tyrosine to compare the degree of its activity inhibition; this method is different from the actual production of melanin in the body, so even if the experimental effect is good, the subsequent practical application The whitening effect after whitening products is often far less than expected

Method used

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  • Phytoextraction composition used for inhibiting tyrosinase and reducing or preventing melanogenesis and pharmaceuticals and application thereof
  • Phytoextraction composition used for inhibiting tyrosinase and reducing or preventing melanogenesis and pharmaceuticals and application thereof
  • Phytoextraction composition used for inhibiting tyrosinase and reducing or preventing melanogenesis and pharmaceuticals and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1. Cell viability test

[0040] The purpose of this example is to confirm the effect of adding plant extracts and inventive compositions on cell survival. In this embodiment, mouse melanoma cells B16-F10 were used, and there were 8 test groups, including control group (DMEM), arbutin (arbutin), resveratrol (resveratrol), curcumin (curcumin) and the present invention. For the compositions AW-001-C2, AW-001-C3, AW-001-C5 and AW-001-C7, each experiment was repeated three times, and the survival rate was analyzed by flow cytometry.

[0041] 1x105 cells were inoculated in a 6-well cell plate (6well plate), and after 24 hours of culture, the composition AW-001 with a concentration of 250ppm arbutin, a concentration of 6ppm resveratrol, a concentration of 8ppm curcumin and a concentration of 8ppm was added respectively -C2, AW-001-C3, AW-001-C5 and AW-001-C7 treated the cells for 48 hours, then collected the cells with Trypsin-EDTA and counted 5x105 cells into a flow ...

Embodiment 2

[0043] Example 2. Inhibition of melanin production experiment (first induce melanin production and then administration)

[0044] This embodiment compares the ability of a single plant extract and the plant extract composition of the present invention to inhibit melanin production. This embodiment uses mouse melanoma cells B16-F10, and there are 8 test groups, which are respectively the control group ( α-MSH), arbutin, resveratrol, curcumin, and compositions of the present invention AW-001-C2, AW-001-C3, AW-001-C5 and AW-001-C7, each group of experiments was repeated 3 times, The melanin content of each group was measured.

[0045] Inoculate 2x105 cells on 6well plate, add 10 nanograms per milliliter (ng / ml) α-MSH for 30 minutes after culturing for 24 hours, and then add 250ppm arbutin and 6ppm to each group except the control group Resveratrol, curcumin at a concentration of 8ppm, and AW-001-C2, AW-001-C3, AW-001-C5, and AW-001-C7 at a concentration of 8ppm were cultured for ...

Embodiment 3

[0047] Example 3. Experiment of inhibiting tyrosinase activity (inducing melanin production before administration)

[0048] In this example, by measuring the amount of L-DOPA converted into dopaquinone, the ability to inhibit tyrosinase activity of a single plant extract and different inventive compositions was evaluated and compared. In this example, mouse melanoma cells B16-F10 were used and divided into 8 groups, namely the control group (α-MSH), arbutin, resveratrol, curcumin and the compositions of the present invention AW-001-C2, AW- 001-C3, AW-001-C5 and AW-001-C7, measure the enzyme activity of plum tyrosine in each group.

[0049] Inoculate 2x105 cells on 6well plate, add 10ng / ml α-MSH for 30 minutes after culturing for 24 hours, and add 250ppm arbutin, 6ppm resveratrol and 8ppm to each group except the control group Curcumin and concentrations of 8ppm AW-001-C2, AW-001-C3, AW-001-C5, AW-001-C7 were cultured for 48 hours, after which the cells were collected with Try...

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PUM

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Abstract

The invention provides a phytoextraction composition used for inhibiting tyrosinase and reducing or preventing melanogenesis and pharmaceuticals and application thereof. The phytoextraction composition comprises curcumin extract and resveratrol according to a weight ratio of (4:1) to (1:4). The phytoextraction composition and the pharmaceuticals thereof disclosed by the invention can be used for preventing, treating and lightening skin color and melanin precipitation, so that the effects of lightening the skin color and reducing the melanin precipitation at local receptor positions are achieved.

Description

technical field [0001] The present invention relates to a plant extract composition, especially a plant extract containing turmeric extract and resveratrol in a specific weight ratio; Medicines for the prevention and treatment of lightening skin color and melanin precipitation; the present invention is more about a use, especially the use of the aforementioned medicines for preventing and treating lightening skin color and melanin precipitation. Background technique [0002] The color of the skin is mainly determined by the content of melanin. Normally, melanin in the skin can be used as a natural protective barrier for the human body. The presence of melanin helps humans resist ultraviolet radiation and avoid photocarcinogenesis. On the contrary, the formation of excessive melanin pigmentation (pigmentation) will cause many troubles, such as age spots, freckles, dark spots, liver spots and other unwanted pigmentation of the skin, or pigmentation produced by the wound healin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/9066A61P17/00A61K31/05
Inventor 凌玉芳
Owner CALIWAY BIOPHARM
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