Phytoextraction composition used for inhibiting tyrosinase and reducing or preventing melanogenesis and pharmaceuticals and application thereof
A technology of melanin and composition, applied in plant extracts of resveratrol, medicines for prevention and treatment of lightening skin color and melanin precipitation, prevention and treatment of lightening skin color and melanin precipitation, can solve the problem of whitening effect not as expected To reduce or prevent melanin production, skin tone or spot removal, and inhibit enzyme activity
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Embodiment 1
[0039] Example 1. Cell viability test
[0040] The purpose of this example is to confirm the effect of adding plant extracts and inventive compositions on cell survival. In this embodiment, mouse melanoma cells B16-F10 were used, and there were 8 test groups, including control group (DMEM), arbutin (arbutin), resveratrol (resveratrol), curcumin (curcumin) and the present invention. For the compositions AW-001-C2, AW-001-C3, AW-001-C5 and AW-001-C7, each experiment was repeated three times, and the survival rate was analyzed by flow cytometry.
[0041] 1x105 cells were inoculated in a 6-well cell plate (6well plate), and after 24 hours of culture, the composition AW-001 with a concentration of 250ppm arbutin, a concentration of 6ppm resveratrol, a concentration of 8ppm curcumin and a concentration of 8ppm was added respectively -C2, AW-001-C3, AW-001-C5 and AW-001-C7 treated the cells for 48 hours, then collected the cells with Trypsin-EDTA and counted 5x105 cells into a flow ...
Embodiment 2
[0043] Example 2. Inhibition of melanin production experiment (first induce melanin production and then administration)
[0044] This embodiment compares the ability of a single plant extract and the plant extract composition of the present invention to inhibit melanin production. This embodiment uses mouse melanoma cells B16-F10, and there are 8 test groups, which are respectively the control group ( α-MSH), arbutin, resveratrol, curcumin, and compositions of the present invention AW-001-C2, AW-001-C3, AW-001-C5 and AW-001-C7, each group of experiments was repeated 3 times, The melanin content of each group was measured.
[0045] Inoculate 2x105 cells on 6well plate, add 10 nanograms per milliliter (ng / ml) α-MSH for 30 minutes after culturing for 24 hours, and then add 250ppm arbutin and 6ppm to each group except the control group Resveratrol, curcumin at a concentration of 8ppm, and AW-001-C2, AW-001-C3, AW-001-C5, and AW-001-C7 at a concentration of 8ppm were cultured for ...
Embodiment 3
[0047] Example 3. Experiment of inhibiting tyrosinase activity (inducing melanin production before administration)
[0048] In this example, by measuring the amount of L-DOPA converted into dopaquinone, the ability to inhibit tyrosinase activity of a single plant extract and different inventive compositions was evaluated and compared. In this example, mouse melanoma cells B16-F10 were used and divided into 8 groups, namely the control group (α-MSH), arbutin, resveratrol, curcumin and the compositions of the present invention AW-001-C2, AW- 001-C3, AW-001-C5 and AW-001-C7, measure the enzyme activity of plum tyrosine in each group.
[0049] Inoculate 2x105 cells on 6well plate, add 10ng / ml α-MSH for 30 minutes after culturing for 24 hours, and add 250ppm arbutin, 6ppm resveratrol and 8ppm to each group except the control group Curcumin and concentrations of 8ppm AW-001-C2, AW-001-C3, AW-001-C5, AW-001-C7 were cultured for 48 hours, after which the cells were collected with Try...
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