A method for rapid detection of main components of khat grass
A fast and fast technology, applied in the field of chemical detection, can solve the problems of small sample volume, long extraction time, and large sample consumption, and achieve stable pretreatment process, short extraction time, and high extraction recovery rate. Effect
Active Publication Date: 2019-02-19
ACADEMY OF FORENSIC SCIENCE
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Problems solved by technology
However, the extraction time of this method is too long and the amount of samples used is large, which cannot meet the situation of small sample amount in actual drug cases and the need for rapid detection when cases are urgent
Method used
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Embodiment 1
[0070] (1) Cut the to-be-inspected chate grass sample into pieces; take the to-be-inspected chate grass sample and place it in a freezing grinding tube, such as figure 1 As shown, a magnetic striker M is added to the freezing grinding tube, and then the freezing grinding tube is immersed in liquid nitrogen, and the magnetic striker M is electromagnetically driven to oscillate at -196° C. Chattergrass sample, prepared Chattergrass powder.
[0071] (2) Place 0.1g of the Chattergrass powder in a container, add 0.5mL of 0.1mol / L hydrochloric acid, sonicate for 30min, then use a 10% NaOH solution to adjust the pH to 12; then add 3mL of ethyl acetate Ester extraction, vortex mixing for 3 minutes, centrifugation at 3000 r / min for 12 minutes, standing, taking the supernatant, drying in a 60°C water bath, and finally adding 0.1 mL of acetonitrile for reconstitution to prepare the sample solution.
[0072] (3) Take 5 μL of the supernatant of the solution to be injected, and inject it into th...
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Abstract
The invention provides a method for rapidly detecting main ingredients of khat, and relates to a method for detecting whether khat exists in a suspicious object or not. The method for rapidly detecting the main ingredients of the khat mainly comprises the following steps that a to-be-detected khat sample is subjected to freeze grinding, and khat powder is obtained; a to-be-injected sample solution is prepared; LC-MS / MS analysis is conducted. By adopting the method for rapidly detecting the main ingredients of the khat, the pretreatment process is stable, and no special requirement is needed; by adopting a freeze grinding technology, the extraction time is short, the extraction recovery rate is high, the to-be-detected khat sample is frozen rapidly in liquid nitrogen and then ground into powder, the main ingredients in the sample can be fully released, rapid grinding and crushing are achieved while the biological activity is kept, and subsequent liquid chromatography tandem mass spectrometry analysis is promoted; the lowest limit of detection of LC-MS / MS analysis is extremely low, and therefore the detection sensitivity is improved, and rapid identification on a suspicious object suspected to be the khat is promoted.
Description
Technical field [0001] The invention belongs to the field of chemical detection, and relates to a method for rapidly detecting the main components of Chattergrass, and also relates to a method for detecting whether there is Chattergrass in suspicious substances. Background technique [0002] Khat, also known as Arabic tea, Yemen tea, Qiaocha, etc., is a plant produced in East Africa and the Arabian Peninsula. The main stimulant ingredients are cathinone and norephedrine (norephedrine), has an exciting effect on the human central nervous system and can make people addicted. In 2013, my country listed Chattergrass as the first class of controlled drugs. According to the relevant provisions of the Chinese Criminal Law, Chattergrass belongs to the category of drugs and it is strictly prohibited to bring it into China. [0003] However, in the actual operation of detecting the main components of Chattergrass, there are often a series of problems. For example, the cathinone in the fresh ...
Claims
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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 施妍向平沈敏卓先义陈航
Owner ACADEMY OF FORENSIC SCIENCE