Adipose mesenchymal stem cell cryoprotectant and method for cryopreservation of adipose mesenchymal stem cells

A technology of quality stem cells and cryopreservation solution, which is applied in the field of stem cell cryopreservation, can solve the problems of damaging stem cells and reducing cell survival rate, and achieve the effects of reducing damage, improving cryopreservation survival rate, and reducing corrosion

Active Publication Date: 2017-01-18
浙江译美生物科技有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0007] In view of the deficiencies in the prior art, the first object of the present invention is to provide a stem cell cryopreservation solution, which solves the problem that the toxicity of dimethyl sulfoxide will damage the stem cells when the stem cells are cryopreserved, thereby reducing the cell survival rate. It has the advantages of not containing dimethyl sulfoxide and good freezing effect

Method used

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  • Adipose mesenchymal stem cell cryoprotectant and method for cryopreservation of adipose mesenchymal stem cells
  • Adipose mesenchymal stem cell cryoprotectant and method for cryopreservation of adipose mesenchymal stem cells
  • Adipose mesenchymal stem cell cryoprotectant and method for cryopreservation of adipose mesenchymal stem cells

Examples

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Embodiment 1

[0068] Embodiment 1: a method for cryopreserving adipose-derived mesenchymal stem cells, comprising the following steps:

[0069] S1, fully mix 1.4 parts of sulfolane, 1.5 parts of 5wt% ethanol, 1 part of 10wt% ethylene glycol, 0.85 parts of 10wt% 1,2-propanediol, 0.4 parts of Tween 80, 0.9 parts of glycerin and 0.6 parts of agar powder to form an auxiliary agent;

[0070] S2, add 80 parts of RPMI-1640 medium, 5 parts of FBS, 0.05 parts of penicillin, 0.8 parts of trehalose, and 0.5 parts of polyvinylpyrrolidone to the auxiliary agent obtained in S1, and mix thoroughly to form a cryopreservation solution mixture;

[0071] S3, taking clinical human abdominal subcutaneous fat tissue, soaking in PBS, washing, centrifuging, and sedimentation to obtain adipose-derived mesenchymal stem cells;

[0072] S4, inoculate and culture the adipose-derived mesenchymal stem cells obtained in S3, take the adipose-derived mesenchymal stem cells in the logarithmic growth phase, wash them and put...

Embodiment 2

[0076] Example 2: A method for cryopreserving adipose-derived mesenchymal stem cells. The difference from Example 1 is that the parts by weight of each component in the adipose-derived mesenchymal stem cell cryopreservation solution are shown in Table 1.

Embodiment 3

[0077] Example 3: A method for cryopreserving adipose-derived mesenchymal stem cells. The difference from Example 2 is that 0.5 parts of hydroxyethyl starch and 0.5 parts of dextran are added to S1 and mixed thoroughly.

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Abstract

The invention discloses an adipose mesenchymal stem cell cryoprotectant and a method for cryopreservation of adipose mesenchymal stem cells. The adipose mesenchymal stem cell cryoprotectant comprises, by weight parts, 80-88 parts of RPMI-1640 culture medium, 2-5 parts of FBS, 1-2 parts of sulfolane, 1.5-2.5 parts of 5 wt% of ethanol, 0.8-1.2 parts of 10 wt% of ethylene glycol, 0.8-1.2 parts of 10 wt% of 1,2-propylene glycol, 0.3-0.7 part of Tween 80, 0.5-1.5 parts of glycerin, 0.01-0.05 part of 50 U/mL of penicillin, 0.5-1 part of trehalose, 0.5-0.8 part of polyvinylpyrrolidone and 0.2-0.6 part of agar powder. The adipose mesenchymal stem cell cryoprotectant has the advantages of having no toxicity to the adipose mesenchymal stem cells and being good in cryopreservation effect.

Description

technical field [0001] The invention relates to the technical field of cryopreservation of stem cells, more specifically, to a cryopreservation solution of adipose-derived mesenchymal stem cells and a method for cryopreservation of adipose-derived mesenchymal stem cells. Background technique [0002] Stem cells are a class of primitive and unspecialized pluripotent cells capable of self-replication. Under certain conditions, it can differentiate into a variety of functional cells. Adipose-derived mesenchymal stem cells are an important type of stem cells, and studies have found that they have immune-regulating effects. [0003] Cell cryopreservation is one of the main methods of cell preservation. The cryopreservation process will significantly change the thermodynamic, chemical and physical environment of cells, with the risk of causing biological damage. The change in temperature mainly depends on the boundary conditions of heat conduction caused by cooling or heating m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 汪铮吴振化
Owner 浙江译美生物科技有限公司
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