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Rapid propagation method for physcomitrella patens protonemata

A technology of Physcomitrella patens and protoceles, applied in the biological field, can solve the problems of low reproductive efficiency and achieve the effects of improving application potential, increasing the number of branches, and increasing biomass

Active Publication Date: 2017-02-01
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
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Problems solved by technology

However, as the main source of transgenic materials, the growth state and quantity of the protonema are the main factors limiting the transgenic efficiency. The reproductive efficiency of the existing technology is low, and there is an urgent need for improved methods to increase the reproductive efficiency.

Method used

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  • Rapid propagation method for physcomitrella patens protonemata
  • Rapid propagation method for physcomitrella patens protonemata
  • Rapid propagation method for physcomitrella patens protonemata

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Embodiment 1

[0021] 1. Materials and methods:

[0022] 1.1 Research materials:

[0023] The materials used in the present invention are Physcomitrella patens GD2004 species, which are stored in a laboratory incubator after multiple generations of plant tissue rapid propagation.

[0024] 1.2 Research methods:

[0025] 1.2.1 Experimental design: The protocelium material of Physcomitrella patens grown for 5 days was mechanically ground and crushed for subculture, and the test plates with different calcium ion concentrations based on BCD medium were set up, and inoculated after grinding and subculture at various concentrations of CaCl 2 On the BCD medium plate, observe the growth state, and finally determine the optimal calcium ion concentration for the growth and differentiation state of the protoceles.

[0026] 1.2.2 Protocelium tissue culture rapid propagation technology: use a homogenizer to grind the plant material mechanically, inoculate the plant homogenate on the medium, place at 23...

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Abstract

The invention provides a rapid propagation method for physcomitrella patens protonemata. Physcomitrella patens protonema materials growing 5 days are subjected to mechanical grinding crushing and subculturing, test plates which take a BCD medium as a basic medium and have different calcium ion concentrations are arranged, the BCD medium plates with different CaCl2 concentrations are inoculated with the protonema materials after the protonema materials are subjected to grinding subculturing, growing status observation is conducted, and finally the calcium ion concentration at which the optimal protonema growing and differentiating states are achieved is determined. A culture and formula improvement experiment for physcomitrella patens protonema culturing shows that on the conditions that the temperature of an incubator is controlled at 23 DEG C, the light intensity is 80 micromoles photons m<-2>s<-1>, and the sun illumination time is 16 hours, the plant protonemata which grow most vigorously and generate the most materials are obtained at the calcium ion concentration of 5-mM, and a technical basis is provided for a later protoplast preparation and conversion recombination experiment.

Description

[0001] Field [0002] The invention belongs to the field of biotechnology, and in particular relates to a method for rapid propagation of Physcomitrella patens protonema tissue culture, in particular to a culture medium for promoting the rapid mass reproduction of Physcomitrella patens protonema. Background technique [0003] Mosses are one of the oldest terrestrial plants, and their evolutionary position is behind algae, before ferns and seed plants, and microtubules belong to sister clades on a single-origin system, which has an extremely important research status (Kidron, 2014 ; Cao et al., 2014). There are about 21,200 species in the world, distributed in every corner of the earth except the ocean, and even grow on deserts, tundra and rocks. They are drought-resistant, cold-resistant, barren-resistant, and highly adaptable. They are the pioneers and pioneers of nature. (Kidron, 2014; Cao Tong et al., 2014). [0004] Physcomitrella patens is a moss of the genus Physcomitr...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 杨红李萍刘莉鲁元学章成君
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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